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Apoptosis induction of oroxylin A in human cervical cancer HeLa cell line in vitro and in vivo
- Source :
- Toxicology. 257(1-2)
- Publication Year :
- 2008
-
Abstract
- Oroxylin A is a flavonoid that is found in the roots of Scutellaria baicalensis Georgi. Here, we investigated the antitumor effect of oroxylin A in human cervical cancer HeLa cell line in vitro and in vivo. We found that after inoculated with the HeLa cells the mice treated with oroxylin A showed a significant decrease of tumor volumes and tumor weight compared with the control. Meanwhile, the growth inhibition of oroxylin A on HeLa cells were observed by MTT assay and the value of IC50 was 19.4 ± 0.7 μM after treatment for 48 h. Upon our previous research, the inhibition by oroxylin A might be through apoptosis. Then apoptosis induced by oroxylin A in HeLa cells was characterized by DAPI staining and Annexin V/PI double staining, and degradation of PARP (poly-ADP–ribose polymerase) was both found in HeLa cells and tumor tissue. Next, activation of the caspase cascade for both the extrinsic and intrinsic pathways were demonstrated in vivo and in vitro, including caspase-8, -9 and -3. We also found that the expression of Bcl-2 protein decreased, which leading to an increase of the Bax/Bcl-2 ratio. Our results showed that oroxylin A exhibited strong antitumor effect in HeLa cell line and apoptosis induction involved in it.
- Subjects :
- Male
Programmed cell death
Pathology
medicine.medical_specialty
Time Factors
Cell Survival
Mice, Nude
Uterine Cervical Neoplasms
Apoptosis
Toxicology
HeLa
chemistry.chemical_compound
Inhibitory Concentration 50
Mice
In vivo
medicine
Animals
Humans
MTT assay
Annexin A5
Cell Shape
Cell Proliferation
bcl-2-Associated X Protein
Flavonoids
Mice, Inbred BALB C
biology
Dose-Response Relationship, Drug
biology.organism_classification
Molecular biology
Antineoplastic Agents, Phytogenic
chemistry
Proto-Oncogene Proteins c-bcl-2
Cell culture
Caspases
Scutellaria baicalensis
Oroxylin A
Female
Poly(ADP-ribose) Polymerases
HeLa Cells
Signal Transduction
Subjects
Details
- ISSN :
- 0300483X
- Volume :
- 257
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Toxicology
- Accession number :
- edsair.doi.dedup.....20be6217a7e2cbd5a74fc0c6d6f1b5e7