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Delivery of plateletTPM3mRNA into breast cancer cells via microvesicles enhances metastasis
- Source :
- FEBS Open Bio, FEBS Open Bio, Vol 9, Iss 12, Pp 2159-2169 (2019)
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Platelets are implicated in the pathophysiology of breast and other cancers through their role in exchanging biomolecules with tumor cells in the tumor microenvironment. Such exchange results in tumor‐educated platelets with altered RNA expression profiles. Multiple lines of evidence indicate that platelet RNA profiles may be suitable as diagnostic biomarkers for cancer‐related biological processes. In this study, we characterized the gene expression signatures of platelets in breast cancer (BC) by high‐throughput sequencing and quantitative real‐time RT‐PCR. Our results indicate that the expression of TPM3 (tropomyosin 3) mRNA is significantly elevated in platelets from patients with BC compared with age‐matched healthy control subjects. Furthermore, up‐regulation of TPM3 mRNA in platelets was found to be significantly correlated with metastasis in patients with BC. Finally, we report that platelet TPM3 mRNA is delivered into BC cells through microvesicles and leads to enhanced migrative phenotype of BC cells. In summary, our findings suggest that the transfer of platelet TPM3 mRNA into cancer cells via microvesicles promotes cancer cell migration, and thus platelet‐derived TPM3 mRNA may be a suitable biomarker for early diagnosis of metastatic BC.<br />Platelets are implicated in the pathophysiology of breast and other cancers through their role in exchanging biomolecules with tumor cells in the tumor microenvironment. In this study, we found that platelet TPM3 (tropomyosin 3) mRNA can be delivered into cancer cells via microvesicles to promote cancer cell migration. Platelet‐derived TPM3 mRNA may be a suitable biomarker for early diagnosis of metastatic breast cancer.
- Subjects :
- Adult
Blood Platelets
0301 basic medicine
Breast Neoplasms
Tropomyosin
General Biochemistry, Genetics and Molecular Biology
Tropomyosin 3
Metastasis
03 medical and health sciences
breast cancer
0302 clinical medicine
Cell Movement
Cell-Derived Microparticles
Cell Line, Tumor
Gene expression
Biomarkers, Tumor
Tumor Microenvironment
medicine
Humans
Platelet
RNA, Messenger
Neoplasm Metastasis
education
lcsh:QH301-705.5
Research Articles
Aged
Neoplastic Processes
platelet
Messenger RNA
Tumor microenvironment
education.field_of_study
Chemistry
Middle Aged
medicine.disease
Microvesicles
Gene Expression Regulation, Neoplastic
TPM3
030104 developmental biology
lcsh:Biology (General)
030220 oncology & carcinogenesis
Cancer cell
Cancer research
biomarker
RNA
Female
Transcriptome
Research Article
Subjects
Details
- ISSN :
- 22115463
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- FEBS Open Bio
- Accession number :
- edsair.doi.dedup.....20ce8c93900044f2857eb372c4aaa52f