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Transcriptome analysis of basal and luminal tumor-initiating cells in ErbB2-driven breast cancer
- Source :
- Genomics Data, Genomics Data, Vol 4, Iss C, Pp 119-122 (2015)
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Breast cancer is the leading cause of cancer-related mortality for females worldwide [1]. Improving early screening strategies and understanding the events that lead to tumor initiation have led to demonstrable improvements in clinical outcome. Our previous work revealed a variance in the tumorigenic capacity between different mammary epithelial cell populations in an MMTV-ErbB2 mouse model. In order to greater understand how different mammary epithelial cells influence the tumorigenic capacity in ErbB2-induced breast cancer, we transplanted different cell populations from pre-neoplastic MMTV-ErbB2 female mice into recipient mice for tumorigenic study. We found that different mammary epithelial cells bear different tumorigenic potentials even when induced by the same ErbB2 proto-oncogene. To understand the difference in tumors formed from different epithelial cells, we performed gene expression profiling using these tumors (GSE64487). Several genes were further validated using real-time reverse transcription polymerase chain reaction (RT-PCR). Here we provide further details on the experimental methods and microarray analysis. This data provides a resource to further understanding how different mammary cell populations can initiate ErbB2-driven tumors and the role of these cell populations as putative tumor-initiating cells (TICs).
- Subjects :
- lcsh:QH426-470
Mammary stem cells
Cell
Luminal progenitors
Tumor initiation
Biology
Biochemistry
Transcriptome
Breast cancer
Data in Brief
Genetics
medicine
Myoepithelial cells
Microarray analysis techniques
Tumor-initiating cells
Myoepithelial cell
medicine.disease
3. Good health
Gene expression profiling
Reverse transcription polymerase chain reaction
lcsh:Genetics
medicine.anatomical_structure
Immunology
Cancer research
Molecular Medicine
Biotechnology
Subjects
Details
- ISSN :
- 22135960
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- Genomics Data
- Accession number :
- edsair.doi.dedup.....20d6946dcff45cf5b0d2d78bf8d2366c
- Full Text :
- https://doi.org/10.1016/j.gdata.2015.04.008