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Introduction of Zinc-salt Fixation for Effective Detection of Immune Cell–related Markers by Immunohistochemistry
- Source :
- Toxicologic pathology, vol 43, iss 6
- Publication Year :
- 2015
- Publisher :
- SAGE Publications, 2015.
-
Abstract
- Tissue localization of immune cells is critical to the study of disease processes in mouse models of human diseases. However, immunohistochemistry (IHC) for immune cell phenotyping in mouse tissue sections presents specific technical challenges. For example, CD4 and CD8 have been difficult to detect using IHC on formalin-fixed and paraffin-embedded mouse tissue, prompting alternative methods. We investigated the use of formalin-free zinc-salt fixation (ZN) and optimized IHC protocols for detecting a panel of immune cell–related markers (CD3, CD4, CD8, Foxp3, B220, F4/80, CD68, and major histocompatibility complex [MHC] class-I, MHC class-II, and Gr-1). The IHC results for these markers were compared on mouse spleen tissue treated with neutral buffered formalin (NBF) or ZN with or ZN without antigen retrieval (AR). Whereas CD4 and CD8 were not detected in NBF-treated tissue, all markers were detected in ZN-treated tissue without AR. Thus, the use of ZN treatment for IHC staining can be a good tool for studying immunoreactive lesions in tissues.
- Subjects :
- CD4-Positive T-Lymphocytes
Pathology
medicine.medical_specialty
Tissue Fixation
Clinical Sciences
zinc-salt fixation
CD8-Positive T-Lymphocytes
Biology
Toxicology
Major histocompatibility complex
Article
Pathology and Forensic Medicine
Mice
Fixatives
chemistry.chemical_compound
immune cells
Immune system
Antigen
Formaldehyde
medicine
Animals
antigen retrieval
formalin fixation
Antigens
Molecular Biology
Immunity, Cellular
Immunity
FOXP3
Cell Biology
Immunohistochemistry
Molecular biology
Staining
Zinc
Emerging Infectious Diseases
Antigen retrieval
chemistry
biology.protein
Cellular
Spleen
Biomarkers
CD8
Subjects
Details
- ISSN :
- 15331601 and 01926233
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- Toxicologic Pathology
- Accession number :
- edsair.doi.dedup.....20dddd9d4a05b287e6f43c69a20fe97e