Characterization of fragment sizes, copy number aberrations and 4‐mer end motifs in cell‐free DNA of hepatocellular carcinoma for enhanced liquid biopsy‐based cancer detection

Authors :: Yang Liu
Jiyan Yu
Shaogui Wan
Hongping Li
Jinliang Xing
Chao Jin
Liping Su
Fei Xu
Jiayun Liu
Dengke Bao
Xiaoming Gu
Xu Guo
Xiaonan Liu
Bo Xu
Qiong Zhang
Xiaohuan Lai
Wenyuan Zheng
Gang Wang
Source :: Molecular Oncology, Molecular Oncology, Vol 15, Iss 9, Pp 2377-2389 (2021)
Publication Year :: 2021
Publisher :: Wiley, 2021.
Abstract: Circulating cell‐free DNA (cfDNA) fragmentomics, which encompasses the measurement of cfDNA length and short nucleotide motifs at the ends of cfDNA molecules, is an emerging field for cancer diagnosis. The utilization of cfDNA fragmentomics for the diagnosis of patients with hepatocellular carcinoma (HCC) caused by hepatitis B virus (HBV) is currently limited. In this study, we utilized whole‐genome sequencing data of cfDNA in samples from patients with HCC (n = 197) and HBV (n = 187) to analyze the association of fragment size selection (<br />Circulating cell‐free DNA (cfDNA) fragmentomics, encompassing the measurement of cfDNA length and short nucleotide motifs at cfDNA ends, is an emerging field in cancer diagnostics. In this study, we utilized whole‐genome sequencing data of cfDNA in patients with hepatocellular carcinoma (HCC). We observed that representative abnormal copy number variation (CNV) alterations and shorter fragment size (

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