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Extract of white sweet potato tuber against TNF-α-induced insulin resistance by activating the PI3K/Akt pathway in C2C12 myotubes
- Source :
- Botanical Studies, Vol 62, Iss 1, Pp 1-11 (2021), Botanical Studies
- Publication Year :
- 2021
- Publisher :
- SpringerOpen, 2021.
-
Abstract
- Background White sweet potato (WSP; Ipomoea batatas L. Simon No. 1) has many potential beneficial effects on metabolic control and diabetes-related insulin resistance. The improvement of insulin resistance by WSP tuber extracts on glucose uptake were not investigated in C2C12 myoblast cells. Results WSP tuberous ethanol extract (WSP-E) was partitioned with ethyl-acetate and water to obtain ethyl-acetate layer (WSP-EA) and water layer (WSP-EW). The WSP-EA shows the highest total phenolic contents and highest antioxidant activity by Folin-Ciocalteu and (2,2-diphenyl-1-picryl-hydrazyl-hydrate, DPPH) assay, respectively. After low concentration horse serum on differentiation inducement of C2C12 myoblasts into mature myotubes, the cells were treated with TNF-α to induce insulin resistance. WSP-EA and WSP-EW extracts increased the uptake of fluorescence glucose analogue (2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino]-2-deoxy-d-glucose, 2-NBDG) in a dose-dependent manner as examined by flow cytometry. The WSP-EA enhanced glucose uptake by activation of phosphorylation of IR (pIR), IRS-1 (pIRS-1) and Akt (pAkt) involved in PI3K (phosphatidylinositol 3-kinase)/protein kinase B (Akt) pathway, also upregulated glucose transporter 4 (GLUT4) expression in myotubes. Conclusions WSP-EA enhanced the glucose uptake in C2C12 myotubes through upregulating the PI3K/Akt pathway. The in vitro data reveal that WSP tuber extracts has potential applications to improve insulin resistance in diabetes.
- Subjects :
- 0301 basic medicine
Glucose uptake
030209 endocrinology & metabolism
Plant Science
Biology
C2C12 myotube
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Insulin resistance
Downregulation and upregulation
medicine
Phosphatidylinositol
Protein kinase B
PI3K/AKT/mTOR pathway
White sweet potato
Glucose transporter
Botany
medicine.disease
Molecular biology
030104 developmental biology
chemistry
2-NBDG
PI3K/Akt pathway
QK1-989
biology.protein
Original Article
GLUT4
Subjects
Details
- Language :
- English
- ISSN :
- 19993110
- Volume :
- 62
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Botanical Studies
- Accession number :
- edsair.doi.dedup.....2160cc853694c01edd2ccccfa2590f3c