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Imperatorin alleviates ROS-mediated airway remodeling by targeting the Nrf2/HO-1 signaling pathway

Authors :
Junfeng Li
Jingzhi Jiang
Mingyu Zheng
Guanghai Yan
Zhemin Xian
Chongyang Wang
Yun Ho Choi
Yan Li
Hongmei Piao
Yuzhe Zhao
Liangchang Li
Source :
Bioscience, biotechnology, and biochemistry. 84(5)
Publication Year :
2020

Abstract

In this study, we investigated the role and mechanism of imperatorin (IMP) in chronic inflammation and airway remodeling. The levels of TNF-α, IL-1β, IL-6, IL-8, VEGF, α-SMA, and ROS were detected by ELISA, immunohistochemistry (IHC), immunofluorescence, and Western blot. In addition, we evaluated the effect of IMP on MAPK, PI3K/Akt, NF-κB, and Nrf2/HO-1 signaling pathways. IMP treatment obviously attenuated the production of inflammatory cytokines and inflammatory cells in bronchoalveolar lavage fluid of OVA-induced airway remodeling model. Meanwhile, it significantly inhibited inflammatory cell infiltration, goblet cell hyperplasia, collagen deposition, VEGF production, α-SMA, and ROS expression. Our study has shown that IMP could regulate the signaling pathways including MAPK, PI3K/Akt, NF-κB, and Nrf2/HO-1 to release the inflammatory responses. IMP might attenuate airway remodeling by the down-regulation of Nrf2/HO-1/ROS/PI3K/Akt, Nrf2/HO-1/ROS/MAPK, and Nrf2/HO-1/ROS/NF-κB signaling pathways. Schematic picture of the role of Imperatorin in alleviating ROS-mediated airway remodeling by targeting the Nrf2/HO-1 signaling pathway.

Details

ISSN :
13476947
Volume :
84
Issue :
5
Database :
OpenAIRE
Journal :
Bioscience, biotechnology, and biochemistry
Accession number :
edsair.doi.dedup.....218e4ebd00dc483ef25861cb225903d1