Back to Search
Start Over
Selenium nanoparticles as new strategy to potentiate γδ T cell anti-tumor cytotoxicity through upregulation of tubulin-α acetylation
Selenium nanoparticles as new strategy to potentiate γδ T cell anti-tumor cytotoxicity through upregulation of tubulin-α acetylation
- Source :
- Biomaterials. 222
- Publication Year :
- 2019
-
Abstract
- Immune cell therapy presents a paradigm for the treatment of malignant tumors. Human Vγ9Vδ2 T cells, a subset of peripheral γδ T cells, have been shown to have promising anti-tumor activity. However, new methodology on how to achieve a stronger anti-tumor activity of Vγ9Vδ2 T cells is under continuous investigation. In this work, we used selenium nanoparticles (SeNPs) to strengthen the anti-tumor cytotoxicity of Vγ9Vδ2 T cells. We found SeNPs pretreated γδ T cells had significantly stronger cancer killing and tumor growth inhibition efficacy when compared with γδ T cells alone. Simultaneously, SeNPs pretreatment could significantly upregulate the expression of cytotoxicity related molecules including NKG2D, CD16, and IFN-γ, meanwhile, downregulate PD-1 expression of γδ T cells. Importantly, we observed that SeNPs promoted tubulin acetylation modification in γδ T cells through interaction between microtubule network and lysosomes since the latter is the primary resident station of SeNPs shown by confocal visualization. In conclusion, SeNPs could significantly potentiate anti-tumor cytotoxicity of Vγ9Vδ2 T cells, and both cytotoxicity related molecules and tubulin acetylation were involved in fine-tuning γδ T cell toxicity against cancer cells. Our present work demonstrated a new strategy for further enhancing anti-tumor cytotoxicity of human Vγ9Vδ2 T cells by using SeNPs-based nanotechnology, not gene modification, implicating SeNPs-based nanotechnology had a promising clinical perspective in the γδ T cell immunotherapy for malignant tumors.
- Subjects :
- Cell Survival
T cell
Biophysics
Bioengineering
Antineoplastic Agents
02 engineering and technology
CD16
Biomaterials
Cell therapy
03 medical and health sciences
Mice
Selenium
Immune system
Tubulin
Cell Line, Tumor
medicine
Animals
Humans
Nanotechnology
Cytotoxicity
030304 developmental biology
0303 health sciences
Chemistry
Receptors, IgG
Acetylation
021001 nanoscience & nanotechnology
NKG2D
medicine.anatomical_structure
Mechanics of Materials
NK Cell Lectin-Like Receptor Subfamily K
Cancer cell
Ceramics and Composites
Cancer research
Nanoparticles
0210 nano-technology
Subjects
Details
- ISSN :
- 18785905
- Volume :
- 222
- Database :
- OpenAIRE
- Journal :
- Biomaterials
- Accession number :
- edsair.doi.dedup.....219e9a7e6181e7cad502ee5527348341