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TheLeptosphaeria maculans ��� Leptosphaeria biglobosaspecies complex in the American continent

Authors :
J. Davey
Hua Li
Onésimo Moreno-Rico
J.P. Didier
Martin J. Barbetti
Lucie Vincenot
J.P. Despeghel
Thierry Rouxel
M. H. Balesdent
C. Wu
Lilian Gout
Azita Dilmaghani
D. Phillips
ONERA - The French Aerospace Lab [Palaiseau]
ONERA
Université de Brest (UBO)
Equipe Image - Laboratoire GREYC - UMR6072
Groupe de Recherche en Informatique, Image, Automatique et Instrumentation de Caen (GREYC)
Université de Caen Normandie (UNICAEN)
Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN)
Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN)
Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)
Dept Microbiol
Universidad Autónoma de Aguascalientes
BIOlogie et GEstion des Risques en agriculture (BIOGER)
AgroParisTech-Institut National de la Recherche Agronomique (INRA)
ONERA-Université Paris Saclay (COmUE)
Groupe de Recherche en Informatique, Image et Instrumentation de Caen (GREYC)
Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN)
Normandie Université (NU)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN)
Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN)
Normandie Université (NU)
Institut National de la Recherche Agronomique (INRA)-AgroParisTech
Source :
Plant Pathology, Plant Pathology, Wiley, 2009, 58 (6), pp.1044-1058. ⟨10.1111/j.1365-3059.2009.02149.x⟩
Publication Year :
2009
Publisher :
Wiley, 2009.

Abstract

International audience; Stem canker of oilseed rape (canola, Brassica napus) is associated with a species complex of two closely related fungal species, Leptosphaeria maculans and L. biglobosa. Of these, L. maculans is the most damaging and develops gene-for-gene relationships with the host. Here, a wide scale analysis of the L. maculans - L. biglobosa species complex was performed throughout the American continent (23 locations from Chile to Canada) plus several locations inWestern Australia for comparison purposes, based on a collection of 1132 isolates from infected tissues of a susceptible cultivar. Fungal species were discriminated on the basis of morphological, phytopathological and molecular criteria and showed that L. biglobosa was closely associated with L. maculans in most of the locations. Multiple gene phylogeny using sequences of ITS, actin and b-tubulin confirmed the prevalence of the L. biglobosa ‘canadensis’ sub-clade in Canada, whereas up to three different sub-clades of L. biglobosa were found in Georgia (USA). Race structure of L. maculans was investigated using a combination of pathogenicity tests and PCR amplification of avirulence alleles AvrLm1, AvrLm4 and AvrLm6. Three contrasting situations were observed: (i) race structure in Ontario, Chile and Georgia was related to that of European and Western Australian populations, with a low race diversity; (ii) only one race was found in Mexico, and not found outside of this country; (iii) a large diversity of races was observed in central Canada (Manitoba, Alberta and Saskatchewan) with very specific features including maintenance of avirulence alleles absent from Europe, absence of the AvrLm7 allele common in Europe (or eastern Canada) and wide location-to-location variability.

Details

ISSN :
13653059 and 00320862
Volume :
58
Database :
OpenAIRE
Journal :
Plant Pathology
Accession number :
edsair.doi.dedup.....21b0305343fd49aa9080111aefdfdff4
Full Text :
https://doi.org/10.1111/j.1365-3059.2009.02149.x