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SKAP interacts with Aurora B to guide end-on capture of spindle microtubules via phase separation
- Source :
- Journal of Molecular Cell Biology
- Publication Year :
- 2021
- Publisher :
- Oxford University Press (OUP), 2021.
-
Abstract
- Chromosome segregation in mitosis is orchestrated by the dynamic interactions between the kinetochore and spindle microtubules. Our recent studies show that mitotic motor CENP-E cooperates with SKAP and forms a link between kinetochore core MIS13 complex and spindle microtubule plus-ends to achieve accurate chromosome alignment in mitosis. However, it remains elusive how SKAP regulates kinetochore attachment from lateral association to end-on attachment during metaphase alignment. Here, we identify a novel interaction between Aurora B and SKAP that orchestrates accurate interaction between the kinetochore and dynamic spindle microtubules. Interestingly, SKAP spontaneously phase-separates in vitro via weak, multivalent interactions into droplets with fast internal dynamics. SKAP and Aurora B form heterogeneous coacervates in vitro, which recapitulate the dynamics and behavior of SKAP comets in vivo. Importantly, SKAP interaction with Aurora B via phase separation is essential for accurate chromosome segregation and alignment. Based on those findings, we reason that SKAP–Aurora B interaction via phase separation constitutes a dynamic pool of Aurora B activity during the lateral to end-on conversion of kinetochore–microtubule attachments to achieve faithful cell division.
- Subjects :
- Cell division
Aurora B kinase
Cell Cycle Proteins
kinase activity
AcademicSubjects/SCI01180
Microtubules
Chromosome segregation
Chromosome Segregation
Genetics
Aurora Kinase B
Humans
Aurora B
Kinase activity
Kinetochores
Molecular Biology
Mitosis
Metaphase
mitosis
Kinetochore
Chemistry
SKAP
Articles
Cell Biology
General Medicine
Cell biology
Spindle apparatus
phase separation
Microtubule-Associated Proteins
HeLa Cells
Subjects
Details
- ISSN :
- 17594685
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Journal of Molecular Cell Biology
- Accession number :
- edsair.doi.dedup.....21b2c3025241e4dca547e5544dbbb970