Post-translational control of the Streptomyces lividans ClgR regulon by ClpP

It has been shown previously that expression of theStreptomyces lividans clpP1P2operon, encoding proteolytic subunits of the Clp complex, theclpC1gene, encoding the ATPase subunit, and thelongene, encoding another ATP-dependent protease, are all activated by ClgR. The ClgR regulon also includes theclgRgene itself. It is shown here that the degradation of ClgR and Lon is ClpP1/P2-dependent and that the two C-terminal alanines of these new substrates are involved in their stability. The ClpC1 protein, which does not end with two alanines, is also accumulated in aclpP1P2mutant. The results presented here support the idea that ClpP1/P2 ensure post-translational control of ClgR regulon members, including ClgR itself.