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MiR-199a-3p affects the multi-chemoresistance of osteosarcoma through targeting AK4
- Source :
- BMC Cancer, Vol 18, Iss 1, Pp 1-9 (2018), BMC Cancer
- Publication Year :
- 2018
- Publisher :
- BMC, 2018.
-
Abstract
- Background MicroRNAs (miRNAs) play vital roles in regulating various biological processes. The dysregulations of miRNAs may result in severe human diseases, including cancer. Methods We performed the qRT-PCR, western blot and the luciferase reporter assays to test whether Adenylate Kinase 4 (AK4) is the target of miR-199a-3p. Up- or down-regulation of miR-199a-3p and/or the AK4 gene was done to detect their roles in OS multi-drug resistance using drug resistance profiling assays. We further predicted the putative signal pathway involved in the miR-199a-3p-mediated OS drug-resistance. Results The AK4 gene is one of the targets of miR-199a-3p and negatively correlates with the effect of miR-199a-3p on OS drug-resistance. In addition, the activity of the NF-кB signaling pathway was drastically altered by the forced changes of the miR-199a-3p level in OS cells. Conclusions Our data revealed that both miR-199a-3p and its target gene AK4 are reversely correlated with the OS drug resistance. Electronic supplementary material The online version of this article (10.1186/s12885-018-4460-0) contains supplementary material, which is available to authorized users.
- Subjects :
- Male
0301 basic medicine
Cancer Research
miR-199a-3p
AK4
Mice, Nude
Adenylate kinase
Bone Neoplasms
Drug resistance
Biology
lcsh:RC254-282
Mice
03 medical and health sciences
0302 clinical medicine
Western blot
Surgical oncology
Cell Line, Tumor
microRNA
Genetics
medicine
Animals
Humans
Gene
Osteosarcoma
medicine.diagnostic_test
Adenylate Kinase
Multi-chemoresistance
medicine.disease
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Drug Resistance, Multiple
Gene Expression Regulation, Neoplastic
MicroRNAs
030104 developmental biology
Oncology
Drug Resistance, Neoplasm
030220 oncology & carcinogenesis
Cancer research
Heterografts
Signal transduction
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 14712407
- Volume :
- 18
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Cancer
- Accession number :
- edsair.doi.dedup.....21dab258b353730ec1cdeb6ea20fd9bd