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Staphylococcus aureus biofilm elicits the expansion, activation and polarization of myeloid-derived suppressor cells in vivo and in vitro
- Source :
- PLoS ONE, Vol 12, Iss 8, p e0183271 (2017), PLoS ONE
- Publication Year :
- 2017
- Publisher :
- Public Library of Science (PLoS), 2017.
-
Abstract
- Staphylococcus aureus (S. aureus) is one of the most common causes of biofilm infections in periprosthetic joint infections (PJIs). Accumulating evidence has shown that the immunosuppressive environment established by S. aureus biofilm infection in PJIs involves the presence of myeloid-derived suppressor cells (MDSCs) and M2-macrophages. Due to the diversity of MDSCs, little is known about whether S. aureus biofilm preferentially expands specific MDSC subsets or whether MDSCs can further differentiate into M2-macrophages during S. aureus biofilm infection. Here, we show that in agreement with the results from an established rat PJI model, S. aureus biofilm cocultured with freshly isolated bone marrow cells (BMCs) in vitro significantly increases the proportions of MDSCs, total macrophages and M2-macrophages. Interestingly, we find that treatment of the BMCs in vitro with S. aureus biofilm preferentially promotes the expansion of monocytic MDSCs but not granulocytic MDSCs. Biofilm treatment also substantially enhances the overall MDSC immunosuppressive activity in addition to the MDSC expansion in vitro. Importantly, we provide evidence that S. aureus biofilm is capable of further stimulating the conversion of monocytic MDSCs into M2-macrophages in vitro and in vivo. Collectively, our studies reveal a direct link between MDSCs and M2-macrophages occurring in S. aureus-associated PJIs.
- Subjects :
- 0301 basic medicine
Male
T-Lymphocytes
Staphylococcus
lcsh:Medicine
medicine.disease_cause
Pathology and Laboratory Medicine
T-Lymphocytes, Regulatory
law.invention
Mice
White Blood Cells
0302 clinical medicine
Spectrum Analysis Techniques
law
Animal Cells
Medicine and Health Sciences
Myeloid Cells
Staphylococcus Aureus
lcsh:Science
Cells, Cultured
Multidisciplinary
Microscopy, Confocal
medicine.diagnostic_test
Chemistry
Reverse Transcriptase Polymerase Chain Reaction
T Cells
Drugs
Flow Cytometry
Immunosuppressives
Bacterial Pathogens
medicine.anatomical_structure
Staphylococcus aureus
Medical Microbiology
Spectrophotometry
Cytophotometry
Pathogens
Cellular Types
Research Article
Immune Cells
Immunology
Bone Marrow Cells
Research and Analysis Methods
Microbiology
Flow cytometry
03 medical and health sciences
In vivo
medicine
Animals
Animal Models of Disease
Microbial Pathogens
Pharmacology
Blood Cells
Bacteria
Macrophages
lcsh:R
Biofilm
Organisms
Biology and Life Sciences
Bacteriology
Cell Biology
biochemical phenomena, metabolism, and nutrition
In vitro
Rats
Mice, Inbred C57BL
Animal Models of Infection
030104 developmental biology
Biofilms
Myeloid-derived Suppressor Cell
Microscopy, Electron, Scanning
Animal Studies
Suppressor
lcsh:Q
Bone marrow
Bacterial Biofilms
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....21e4666019c906114963fb8bed8e254e