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Comparison of cyclin A and MIB-1 expression in astrocytic tumors using image-based cell analysis system
- Source :
- Applied immunohistochemistrymolecular morphology : AIMM. 9(1)
- Publication Year :
- 2001
-
Abstract
- Traditional prognostic indicators for astrocytic tumors include tumor size, type, and histologic grade. Data suggest that tumor growth fraction assessed by MIB-1 is an important predicator of survival. Cyclin A, like MIB-1, is a recently described specific marker of proliferation, detectable primarily in S phase of the cell cycle as it undergoes progression to G2 phase. Thirty-seven cases of astrocytic tumors--14 cases of World Health Organization grade 1 and 2 (low grade tumors), 8 cases of grade 3 (anaplastic astrocytoma), and 15 cases of grade 4 (glioblastoma multiforme)--were simultaneously evaluated using routine paraffin immunohistochemical methods with commercial antibodies against MIB-1 and cyclin A. The results were quantitated using a Cell Analysis System (CAS) 200 image analyzer. The mean percentage positive nuclear area for MIB-1 was 3.32% in grade 1 and 2, 19.27% in grade 3, and 24.00% in grade 4 astrocytic tumors. Cyclin A showed a similar pattern of positivity in the same cases: 2.84% in grade 1 and 2, 16.27% in grade 3, and 24.88% in grade 4 astrocytic tumors. The data suggest that both proliferation markers correlated significantly with histologic grade. Cyclin A appears to be as good an indicator of brain tumor proliferation as MIB-1. Because cyclin A is detectable primarily in the S phase of the cell cycle, the fraction of cells positive for cyclin A should allow for a more accurate indicator of tumor progression.
- Subjects :
- Pathology
medicine.medical_specialty
Histology
Cyclin A
Brain tumor
Astrocytoma
Pathology and Forensic Medicine
medicine
Biomarkers, Tumor
Image Processing, Computer-Assisted
Humans
S phase
Cyclin
Retrospective Studies
biology
business.industry
Brain Neoplasms
Cell Cycle
Nuclear Proteins
Antigens, Nuclear
Cell cycle
medicine.disease
Prognosis
Immunohistochemistry
Medical Laboratory Technology
Ki-67 Antigen
Tumor progression
biology.protein
Anatomy
business
Glioblastoma
Anaplastic astrocytoma
Subjects
Details
- ISSN :
- 15412016
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Applied immunohistochemistrymolecular morphology : AIMM
- Accession number :
- edsair.doi.dedup.....21f15a21a4c8bb5e3c542ac1ac944315