Back to Search
Start Over
Oxidative stress induces gastric submucosal arteriolar dysfunction in the elderly
- Source :
- World journal of gastroenterology. 19(48)
- Publication Year :
- 2013
-
Abstract
- AIM: To evaluate human gastric submucosal vascular dysfunction and its mechanism during the aging process. METHODS: Twenty male patients undergoing subtotal gastrectomy were enrolled in this study. Young and elderly patient groups aged 25-40 years and 60-85 years, respectively, were included. Inclusion criteria were: no clinical evidence of cardiovascular, renal or diabetic diseases. Conventional clinical examinations were carried out. After surgery, gastric submucosal arteries were immediately dissected free of fat and connective tissue. Vascular responses to acetylcholine (ACh) and sodium nitroprusside (SNP) were measured by isolated vascular perfusion. Morphological changes in the gastric mucosal vessels were observed by hematoxylin and eosin (HE) staining and Verhoeff van Gieson (EVG) staining. The expression of xanthine oxidase (XO) and manganese-superoxide dismutase (Mn-SOD) was assessed by Western blotting analysis. The malondialdehyde (MDA) and hydrogen peroxide (H2O2) content and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were determined according to commercial kits. RESULTS: The overall structure of vessel walls was shown by HE and EVG staining, respectively. Disruption of the internal elastic lamina or neointimal layers was not observed in vessels from young or elderly patients; however, cell layer number in the vessel wall increased significantly in the elderly group. Compared with submucosal arteries in young patients, the amount of vascular collagen fibers, lumen diameter and media cross-sectional area were significantly increased in elderly patients. Ach- and SNP-induced vasodilatation in elderly arterioles was significantly decreased compared with that of gastric submucosal arterioles from young patients. Compared with the young group, the expression of XO and the contents of MDA and H2O2 in gastric submucosal arterioles were increased in the elderly group. In addition, the expression of Mn-SOD and the activities of SOD and GSH-Px in the elderly group decreased significantly compared with those in the young group. CONCLUSION: Gastric vascular dysfunction and senescence may be associated with increased oxidative stress and decreased antioxidative defense in the aging process.
- Subjects :
- Adult
Male
medicine.medical_specialty
Aging
Xanthine Oxidase
Brief Article
Vasodilator Agents
H&E stain
Connective tissue
Vasodilation
medicine.disease_cause
Gastroenterology
chemistry.chemical_compound
Internal medicine
Malondialdehyde
medicine
Humans
Aged
chemistry.chemical_classification
Aged, 80 and over
Glutathione Peroxidase
business.industry
Superoxide Dismutase
Glutathione peroxidase
digestive, oral, and skin physiology
Age Factors
General Medicine
Hydrogen Peroxide
Middle Aged
Internal elastic lamina
digestive system diseases
Surgery
Arterioles
Oxidative Stress
medicine.anatomical_structure
chemistry
Gastric Mucosa
business
Perfusion
Oxidative stress
Subjects
Details
- ISSN :
- 22192840
- Volume :
- 19
- Issue :
- 48
- Database :
- OpenAIRE
- Journal :
- World journal of gastroenterology
- Accession number :
- edsair.doi.dedup.....2206a117ca3305d685f74440de13bb0f