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Sulphonated Formononetin Induces Angiogenesis through Vascular Endothelial Growth Factor/cAMP Response Element-Binding Protein/Early Growth Response 3/Vascular Cell Adhesion Molecule 1 and Wnt/β-Catenin Signaling Pathway
- Source :
- Pharmacology. 101(1-2)
- Publication Year :
- 2017
-
Abstract
- Background: Sodium formononetin-3’-sulphonate (Sul-F) is a derivative of the isoflavone formononetin. In this study, we investigated whether Sul-F can regulate angiogenesis and the potential mechanism in vitro. Methods: We examined the effects of Sul-F on cell proliferation, cell invasion, and tube formation in the human umbilical vein endothelial cell line (HUVEC). To better understand the mechanism involved, we investigated effects of the following compounds: cAMP response element-binding protein (CREB) inhibitor 2-naphthol-AS-E-phosphate (KG-501), early growth response 3 (Egr-3) siRNA, vascular endothelial growth factor (VEGF) antagonist soluble VEGF receptor 1 (sFlt-1), VEGF receptor 2 blocker SU-1498, Wnt5a antagonist WIF-1 recombinant protein (WIF-1), and inhibitor of Wnt/β-catenin recombinant Dickkopf-1 protein (DKK-1). HUVEC proliferation was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). A scratch adhesion test was used to assess cell invasion ability. Matrigel tube formation assay was performed to test capillary tube formation ability. Activation of the VEGF/CREB/Egr-3/Vascular cell adhesion molecule 1 (VCAM-1) pathway in HUVEC was tested by Western blot analysis. Results: Our results suggest that Sul-F induced angiogenesis in vitro by enhancing cell proliferation, invasion, and tube formation. The increase in proliferation and tube formation by Sul-F was counteracted by DKK-1, WIF-1, SU1498, KG-501, sFlt-1, and Egr-3 siRNA. Conclusions: These results may suggest that Sul-F induces angiogenesis in vitro via a programed Wnt/β-catenin pathway and VEGF/CREB/Egr-3/VCAM-1 signaling axis.
- Subjects :
- 0301 basic medicine
Vascular Endothelial Growth Factor A
Angiogenesis
Cell Survival
Neovascularization, Physiologic
Vascular Cell Adhesion Molecule-1
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Cell Movement
Human Umbilical Vein Endothelial Cells
Humans
Cyclic AMP Response Element-Binding Protein
Wnt Signaling Pathway
Cells, Cultured
Cell Proliferation
Pharmacology
Tube formation
L-Lactate Dehydrogenase
Cell adhesion molecule
Wnt signaling pathway
General Medicine
Isoflavones
Cell biology
Vascular endothelial growth factor
Vascular endothelial growth factor B
Vascular endothelial growth factor A
030104 developmental biology
chemistry
Vascular endothelial growth factor C
Biochemistry
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 14230313
- Volume :
- 101
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Pharmacology
- Accession number :
- edsair.doi.dedup.....22133ce318cf1cc1b882eaac305fdf92