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X-ray spectroscopy and X-ray diffraction at wavelengths near theK-absorption edge of phosphorus
- Source :
- Journal of Synchrotron Radiation, Journal of Synchrotron Radiation, International Union of Crystallography, 2005, 12 (Pt 4), pp.402-9. ⟨10.1107/S0909049505009222⟩, Journal of Synchrotron Radiation, 2005, 12 (Pt 4), pp.402-9. ⟨10.1107/S0909049505009222⟩
- Publication Year :
- 2005
- Publisher :
- International Union of Crystallography (IUCr), 2005.
-
Abstract
- International audience; Phosphorus is an abundant element in living organisms. It is traceable by its X-ray absorption spectrum which shows a strong white line at its K-edge, comparable with that observed for the L(III) edges of rare earth ions. With purple membrane, the variation of the imaginary part of the anomalous dispersion of phosphorus is found to be close to 20 anomalous electron units. Anomalous diffraction experiments at wavelengths near the K-absorption edge of phosphorus confirm this result. The spatial distribution of lipids derived from anomalous diffraction agrees with earlier results from neutron diffraction. Test experiments on single crystals of the carrier protein using 5.76 A photons gave a first low-resolution diffraction pattern. Various techniques of crystal mounting were attempted. In addition, fluorescence measurements on a solution of threonine synthase appear to hint at a change of the phosphate environment of the cofactor upon activator binding.
- Subjects :
- Halobacterium
Diffraction
Nuclear and High Energy Physics
Absorption spectroscopy
Protein Conformation
Carbon-Oxygen Lyases
Neutron diffraction
Molecular Conformation
Analytical chemistry
MESH: Equipment Failure Analysis
01 natural sciences
MESH: Carbon-Oxygen Lyases
Crystal
03 medical and health sciences
MESH: Protein Conformation
Purple Membrane
X-Ray Diffraction
0103 physical sciences
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM]
MESH: Phosphorus
Instrumentation
030304 developmental biology
010302 applied physics
MESH: Molecular Conformation
0303 health sciences
X-ray spectroscopy
Radiation
Chemistry
MESH: X-Ray Diffraction
Spectrometry, X-Ray Emission
MESH: Spectrometry, X-Ray Emission
Phosphorus
Equipment Design
MESH: Purple Membrane
Equipment Failure Analysis
Wavelength
Crystallography
Absorption edge
X-ray crystallography
MESH: Halobacterium
MESH: Mitochondrial ADP, ATP Translocases
Mitochondrial ADP, ATP Translocases
MESH: Equipment Design
Subjects
Details
- ISSN :
- 09090495 and 16005775
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of Synchrotron Radiation
- Accession number :
- edsair.doi.dedup.....223028ed5fc90f6904f0d40d79e642f3
- Full Text :
- https://doi.org/10.1107/s0909049505009222