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Neuroendocrine Cells in Diffuse Gastric Carcinomas
- Source :
- Applied Immunohistochemistry & Molecular Morphology. 18:62-68
- Publication Year :
- 2010
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2010.
-
Abstract
- Neuroendocrine differentiation is often found in gastric carcinomas, but the relevance of these cells in gastric carcinogenesis is debated. We applied immunolabeling at the electron microscopic level to study the ultrastructure of neuroendocrine cells in gastric carcinomas to ensure correct cellular classification of dedifferentiated cells. The immunogold labeling at electron microscopic level was compared with an established sensitive immunohistochemical method using light microscopy. Thirteen human gastric adenocarcinomas of the diffuse type were examined for neuroendocrine differentiation by chromogranin A (CgA) labeling at both the light and electron microscopic level. The ultrastructure of CgA-positive cells was compared with CgA-positive cells from controls. Nine of 13 tumors showed CgA-positive cells both at the light and electron microscopic level. The CgA-positive cells displayed altered ultrastructural features compared with controls. Some of the CgA-positive tumor cells had granules typical for enterochromaffin-like cells. Immunoelectron microscopy seems to provide both significant immunolabeling and sufficient ultrastructure to enhance classification of cells in neoplastic tissue.
- Subjects :
- Oncology
endocrine system
medicine.medical_specialty
Pathology
Histology
Enterochromaffin-like Cells
Immunoelectron microscopy
Neuroendocrine differentiation
Pathology and Forensic Medicine
Immunolabeling
Neuroendocrine Cells
Stomach Neoplasms
Internal medicine
medicine
Humans
Neoplastic tissue
biology
Chemistry
Chromogranin A
Immunogold labelling
Immunohistochemistry
Microscopy, Electron
Medical Laboratory Technology
Case-Control Studies
Ultrastructure
biology.protein
Subjects
Details
- ISSN :
- 15412016
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Applied Immunohistochemistry & Molecular Morphology
- Accession number :
- edsair.doi.dedup.....2276eb04f5198810568bc8f7e7f138b3