Back to Search
Start Over
Pharmacological postconditioning with tanshinone IIA attenuates myocardial ischemia-reperfusion injury in rats by activating the phosphatidylinositol 3-kinase pathway
- Source :
- Experimental and Therapeutic Medicine
- Publication Year :
- 2014
- Publisher :
- D.A. Spandidos, 2014.
-
Abstract
- Tanshinone IIA, one of the active ingredients in the Chinese medicine Danshen, is cardioprotective when applied prior to sustained myocardial ischemia. The present study aimed to investigate whether pharmacological postconditioning with tanshinone IIA attenuates myocardial ischemia-reperfusion injury when applied prior to prolonged reperfusion following a sustained ischemia. A total of 88 Sprague-Dawley rats received 30 min myocardial ischemia followed by 5 or 120 min reperfusion. Compared with the ischemia-reperfusion model group, the group that received an intravenous injection of 10 mg/kg tanshinone IIA prior to reperfusion had a reduced myocardial infarct size, higher levels of phospho-Akt and phospho-endothelial nitric oxide synthase and less reduction in the optical density of the mitochondria at 540 nm, indicating that the mitochondrial permeability transition (MPT) was attenuated. The cardioprotective effect conferred by tanshinone IIA was abolished by LY294002, a specific inhibitor of phosphatidylinositol 3-kinase (PI3K). These results demonstrate that tanshinone IIA postconditioning protects the myocardium from ischemia-reperfusion injury through the PI3K/Akt pathway, and the MPT may be also involved in this process.
- Subjects :
- Cancer Research
medicine.medical_specialty
phosphatidylinositol 3-kinase
Ischemia
Pharmacology
postconditioning
chemistry.chemical_compound
Immunology and Microbiology (miscellaneous)
Medicine
LY294002
Phosphatidylinositol
PI3K/AKT/mTOR pathway
biology
business.industry
General Medicine
Articles
medicine.disease
reperfusion injury
tanshinone IIA
Surgery
Nitric oxide synthase
myocardial infarction
Mitochondrial permeability transition pore
chemistry
Apoptosis
biology.protein
business
Reperfusion injury
mitochondrial permeability transition
Subjects
Details
- Language :
- English
- ISSN :
- 17921015 and 17920981
- Volume :
- 8
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Experimental and Therapeutic Medicine
- Accession number :
- edsair.doi.dedup.....22857c4a28e6f62f6762a0e2ea1a48b7