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STIM1 is required for Ca2+ signaling during mammalian fertilization
- Source :
- Developmental biology. 367(2)
- Publication Year :
- 2012
-
Abstract
- During fertilization in mammals, a series of oscillations in the oocyte's intracellular free Ca(2+) concentration is responsible for oocyte activation and stimulation of embryonic development. The oscillations are associated with influx of Ca(2+) across the plasma membrane that is probably triggered by the depletion of the intracellular stores, a mechanism known as store-operated Ca(2+) entry. Recently, STIM1 has been identified in oocytes as a key component of the machinery that generates the Ca(2+) influx after store depletion. In this study, the involvement of STIM1 in the sperm-induced Ca(2+) oscillations and its significance in supporting subsequent embryo development were investigated. Downregulation of STIM1 levels in pig oocytes by siRNA completely inhibited the repetitive Ca(2+) signal triggered by the fertilizing sperm. In addition, a significantly lower percentage of oocytes cleaved or formed blastocysts when STIM1 was downregulated prior to fertilization compared to the control groups. Restoring STIM1 levels after fertilization in such oocytes by means of mRNA injection could not rescue embryonic development that in most cases was arrested at the 2-cell stage. On the other hand, STIM1 overexpression prior to fertilization did not alter the pattern of sperm-induced Ca(2+) oscillations and development of these fertilized oocytes up to the blastocyst stage was also similar to that registered in the control group. Finally, downregulation of STIM1 had no effect on oocyte activation when activation was stimulated artificially by inducing a single large elevation in the oocyte's intracellular free Ca(2+) concentration. These findings suggest that STIM1 is essential for normal fertilization as it is involved in the maintenance of the long-lasting repetitive Ca(2+) signal.
- Subjects :
- inorganic chemicals
Male
medicine.medical_specialty
STIM1
Swine
Recombinant Fusion Proteins
Sus scrofa
Down-Regulation
Embryonic Development
Biology
In Vitro Techniques
Embryo development
Human fertilization
Downregulation and upregulation
Internal medicine
medicine
Animals
Humans
Blastocyst
Calcium Signaling
Stromal Interaction Molecule 1
RNA, Small Interfering
Molecular Biology
DNA Primers
Membrane Glycoproteins
Base Sequence
Embryogenesis
Membrane Proteins
Oocyte activation
Ca2+ oscillation
Cell Biology
Oocyte
Sperm
Cell biology
Neoplasm Proteins
medicine.anatomical_structure
Endocrinology
Store-operated Ca2+ entry
Fertilization
Oocytes
Female
Intracellular
Developmental Biology
Subjects
Details
- ISSN :
- 1095564X
- Volume :
- 367
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Developmental biology
- Accession number :
- edsair.doi.dedup.....2290cb89ea4f5bda23104e2824a07363