Rapid Prototyping of Patterned Poly-L-Lysine Microstructures

For applications in cell biology, the ability to produce patterns of adhesion proteins for directing cell patterning is of particular interest. Often though, these patterns require extensive clean room facilities and intricate chrome masks to achieve very small feature sizes. We have developed a modified lift-off method for rapid prototyping of simple PLL structures that have features on a micron scale. The lift-off method is simple and easily adaptable to a variety of biological applications.