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Genetic diversity of staphylococcus aureus influences disease phenotype of systemic lupus erythematosus
- Publication Year :
- 2021
- Publisher :
- Published by Oxford University Press on behalf of the British Society for Rheumatology, 2021.
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Abstract
- Objective We investigated the genetic diversity, molecular epidemiology and evolutionary dynamics of Staphylococcus aureus (SA) isolated from SLE patients by means of phylogenetic analysis. Methods Consecutive SLE patients (ACR 1997 criteria) were enrolled: clinical/laboratory data were collected and nasal swab for SA identification was performed. On the basis of the translation elongation factor (tuf) gene, a phylogenetic analysis was performed to investigate relationships and to assess significant clades. Selective pressure analysis was used to investigate the evolution of the SA tuf gene. The gene sequences from non-SLE individuals, downloaded from the GenBank database, were compared through phylogenetic analysis with the tuf gene from SLE patients. Results We enrolled 118 patients [M/F 10/108; median (interquartile range (IQR)) age 45.5 (13.2) years; median (IQR) disease duration 120 (144) months]. Twenty-four patients (20.3%) were SA carriers (SA+), three of them MRSA. SA+ SLE showed significantly higher SLEDAI-2k values [SA+: median (IQR) 2 (3.75); SA−: 0 (2); P = 0.04]. The phylogenetic analysis, restricted to 21 non-MRSA SA+, revealed a statistically supported larger clade (A, n = 17) and a smaller one (B, n = 4). Patients located in clade A showed a significantly higher prevalence of joint involvement (88.2%) in comparison with clade B (50.0%, P Conclusion We suggest a possible role of SA nasal carriage status in SLE disease activity. Moreover, our findings support the hypothesis that bacterial genetic variants may be associated with specific disease features.
- Subjects :
- 0301 basic medicine
Male
staphylococcus aureus
medicine.medical_specialty
disease phenotype
medicine.disease_cause
Gastroenterology
Pathogenesis
03 medical and health sciences
0302 clinical medicine
Rheumatology
systemic lupus erythematosus
Interquartile range
Internal medicine
medicine
Humans
Lupus Erythematosus, Systemic
Pharmacology (medical)
Clade
Correlation of Data
Phylogeny
030203 arthritis & rheumatology
Genetic diversity
Molecular epidemiology
Phylogenetic tree
business.industry
pathogenesis
phylogenetic analysis
Immunity
Patient Acuity
Genetic Variation
Middle Aged
Staphylococcal Infections
Methicillin-resistant Staphylococcus aureus
030104 developmental biology
Italy
Staphylococcus aureus
Genes, Bacterial
Female
Joint Diseases
Nasal Cavity
Symptom Assessment
business
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....22bf5f85414c0547477a37ec18c93451