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Generation of three human induced pluripotent stem cell lines with IRX5 knockout and knockin genetic editions using CRISPR-Cas9 system
- Source :
- Stem cell research, 58:102627. Elsevier, Stem Cell Research, Vol 58, Iss, Pp 102627-(2022), Stem Cell Research, Stem Cell Research, 2022, 58, pp.102627. ⟨10.1016/j.scr.2021.102627⟩
- Publication Year :
- 2022
- Publisher :
- Elsevier BV, 2022.
-
Abstract
- Studies on animal models have shown that Irx5 is an important regulator of cardiac development and that it regulates ventricular electrical repolarization gradient in the adult heart. Mutations in IRX5 have also been linked in humans to cardiac conduction defects. In order to fully characterize the role of IRX5 during cardiac development and in cardiomyocyte function, we generated three genetically-modified human induced pluripotent stem cell lines: two knockout lines (heterozygous and homozygous) and a knockin HA-tagged line (homozygous).<br />French National Research Agency; La Fédération Française de Cardiologie; Fondation Lefoulon-Delalande; Marie Curie Inter-national Incoming Fellowship FP7-PEOPLE-2012-IIF; Marion Elizabeth Brancher (MEB)
- Subjects :
- Homeodomain Proteins
Heterozygote
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
QH301-705.5
Homozygote
Induced Pluripotent Stem Cells
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Cell Biology
General Medicine
Homeobox genes
Transcription Factor
Liver cell carcinoma
[SDV.BIO] Life Sciences [q-bio]/Biotechnology
Genetics
cardiovascular system
Animals
Humans
Myocytes, Cardiac
Biology (General)
CRISPR-Cas Systems
[SDV.BC] Life Sciences [q-bio]/Cellular Biology
Transcription Factors
Developmental Biology
Subjects
Details
- ISSN :
- 18735061 and 18767753
- Volume :
- 58
- Database :
- OpenAIRE
- Journal :
- Stem Cell Research
- Accession number :
- edsair.doi.dedup.....22d3d80425ea1256d4152f4840549d0e