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Crystal structures of an ICAM-5 ectodomain fragment show electrostatic-based homophilic adhesions
- Source :
- 'Acta Crystallographica D ', vol: 70, pages: 1934-1943 (2014), Acta Crystallographica Section D: Biological Crystallography
- Publication Year :
- 2014
-
Abstract
- Crystal structures of Intercellular Cell Adhesion Molecule-5 (ICAM-5) show charge-based homotypic interactions and a potential ICAM-5 cell adhesion complex in neurons.<br />Intercellular cell adhesion molecule-5 (ICAM-5) is a member of the ICAM subfamily that is exclusively expressed in the telencephalon region of the brain. The crystal structure of the four most N-terminal glycosylated domains (D1–D4) of ICAM-5 was determined in three different space groups and the D1–D5 fragment was modelled. The structures showed a curved molecule with two pronounced interdomain bends between D2 and D3 and between D3 and D4, as well as some interdomain flexibility. In contrast to ICAM-1, ICAM-5 has patches of positive and negative electrostatic charge at D1–D2 and at D3–D5, respectively. ICAM-5 can mediate homotypic interactions. In the crystals, several charge-based intermolecular interactions between the N-terminal and C-terminal moieties of the ICAM-5 molecules were observed, which defined an interacting surface in the D1–D4 fragment. One of the crystal lattices has a molecular assembly that could represent the homophilic ICAM-5 cell adhesion complex in neurons.
- Subjects :
- ICAM-5
Protein Conformation
Molecular Sequence Data
Static Electricity
Nerve Tissue Proteins
Crystal structure
immunoglobulin superfamily
Crystallography, X-Ray
Protein structure
Structural Biology
Static electricity
Humans
Amino Acid Sequence
Cell adhesion
Sequence Homology, Amino Acid
Chemistry
Cell adhesion molecule
Intermolecular force
cell adhesion
General Medicine
Research Papers
neuron
Crystallography
Ectodomain
Immunoglobulin superfamily
Cell Adhesion Molecules
Subjects
Details
- Language :
- English
- ISSN :
- 09074449
- Volume :
- 70
- Database :
- OpenAIRE
- Journal :
- Acta Crystallographica D
- Accession number :
- edsair.doi.dedup.....22df092bd141648b431f90f16b6c9b9d