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Optimum conditions for electric pulse-mediated gene transfer to mammalian cells in suspension
- Source :
- Cell Structure and Function. 12:173-180
- Publication Year :
- 1987
- Publisher :
- Japan Society for Cell Biology, 1987.
-
Abstract
- A pulse-generating machine which delivers exponentially decaying pulses over broad range of pulse lengths was used to determine the optimum pulse conditions for gene transfer to FM3A cells. In the transformation of tk- cells with pTK1, a single pulse of 100-2000 microseconds gave a high transformation frequency at 1.5-6 kV/cm and room temperature, the highest transformation frequency obtained being 3 X 10(-3). As the suspension buffer for cells exposed to the pulse, Saline G was better than PBS(-) for obtaining a large number of transformants because it ensured high cell viability.
- Subjects :
- Range (particle radiation)
Materials science
Cell Survival
Physiology
Pulse (signal processing)
Electroporation
Genetic transfer
Analytical chemistry
Cell Biology
General Medicine
Thymidine Kinase
Electric Stimulation
Cell Line
Mice
Transformation (genetics)
Microsecond
Cell culture
Animals
Cloning, Molecular
Suspension (vehicle)
Molecular Biology
Plasmids
Subjects
Details
- ISSN :
- 13473700 and 03867196
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Cell Structure and Function
- Accession number :
- edsair.doi.dedup.....23171f1d5c398d81c463acaee3f20b04