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Ultratrace LC-MS/MS Analysis of Segmented Calf Hair for Retrospective Assessment of Time of Clenbuterol Administration in Agriforensics
- Source :
- Journal of Agricultural and Food Chemistry, 63(2), 493-499, Journal of Agricultural and Food Chemistry 63 (2015) 2
- Publication Year :
- 2015
-
Abstract
- In agriforensics, time of administration is often debated when illegal drug residues, such as clenbuterol, are found in frequently traded cattle. In this proof-of-concept work, the feasibility of obtaining retrospective timeline information from segmented calf tail hair analyses has been studied. First, an ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) hair analysis method was adapted to accommodate smaller sample sizes and in-house validated. Then, longitudinal 1 cm segments of calf tail hair were analyzed to obtain clenbuterol concentration profiles. The profiles found were in good agreement with calculated, theoretical positions of the clenbuterol residues along the hair. Following assessment of the average growth rate of calf tail hair, time of clenbuterol administration could be retrospectively determined from segmented hair analysis data. The data from the initial animal treatment study (n = 2) suggest that time of treatment can be retrospectively estimated with an error of 3-17 days.
- Subjects :
- Time Factors
Novel Foods & Agrochains
BU Toxicologie
Tandem mass spectrometry
Novel Foods & Agroketens
BU Dierbehandelingsmiddelen
BU Veterinary Drugs
calves
beta-agonist residues
contamination
Tandem Mass Spectrometry
human urine
Lc ms ms
Adrenergic beta-Agonists
medicine
Animals
tandem mass-spectrometry
Clenbuterol
samples
BU Toxicology, Novel Foods & Agrochains
Directie
Growth Substances
Chromatography, High Pressure Liquid
Retrospective Studies
VLAG
performance liquid-chromatography
Chromatography
Chemistry
Hair analysis
BU Toxicology
Organic Chemistry
Veterinary Drugs
General Chemistry
Organische Chemie
Drug Residues
BU Toxicologie, Novel Foods & Agroketens
cattle
Treatment study
bovine hair
equine hair
General Agricultural and Biological Sciences
Hair
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 00218561
- Volume :
- 63
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry
- Accession number :
- edsair.doi.dedup.....2320b6a61b9cb1cbdf8c3106efdbf45d