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Postembedding immunocytochemical GABA labeling in rat neocortex cultures: applicability in quantitative studies
- Source :
- Neuroscience Letters, 75, 23-30. Elsevier Ireland Ltd
- Publication Year :
- 1987
-
Abstract
- The value of γ-aminobutyric acid (GABA) immunogold labeling in the quantification of nerve endings was studied in dissociated rat neocortex cultures. Adjacent sections were processed in 3 series according to the same staining protocol. Of all nerve ending profiles examined, 20% appeared to be GABA-positive, while 7% could not be classified. In cultures pretreated with gabaculine, GABA-positive endings were labeled more heavily. Cell bodies could always be discriminated as GABA-positive or GABA-negative. Of all neuronal cell profiles, 14% appeared to be GABA-positive. These neurons could also be identified by light microscopy using the peroxidase-anti-peroxidase method.
- Subjects :
- Cyclohexanecarboxylic Acids
Biology
gamma-Aminobutyric acid
chemistry.chemical_compound
Gabaculine
Culture Techniques
mental disorders
Immunochemistry
medicine
Animals
gamma-Aminobutyric Acid
Cerebral Cortex
Nerve Endings
Neocortex
Histocytochemistry
General Neuroscience
Immunogold labelling
Molecular biology
Staining
Rats
Microscopy, Electron
medicine.anatomical_structure
nervous system
chemistry
Biochemistry
Cerebral cortex
Free nerve ending
medicine.drug
Subjects
Details
- ISSN :
- 03043940
- Volume :
- 75
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Neuroscience letters
- Accession number :
- edsair.doi.dedup.....232440132ce7d0fe6b22d80470ed2f1c