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Possible involvement of cyclic AMP and calcium ion in prostaglandin E1-induced elevation of c-myc mRNA levels in Swiss 3T3 fibroblasts
- Source :
- Journal of Biological Chemistry. 261:16878-16882
- Publication Year :
- 1986
- Publisher :
- Elsevier BV, 1986.
-
Abstract
- Prostaglandin E1 (PGE1) caused a rapid and dose-dependent increase in cAMP levels, followed by elevation of c-myc mRNA levels and then increased DNA synthesis in quiescent cultures of Swiss 3T3 fibroblasts. The dose-response curves of PGE1 were nearly the same for each of these three processes. Both 8-bromo-cAMP and forskolin increased c-myc mRNA levels to 40-50% and DNA synthesis to 70-80% of those caused by a maximally effective dose of PGE1. Under the comparable conditions, PGE1 did not stimulate diacylglycerol formation or activate protein kinase C. However, PGE1 did elevate cytoplasmic free Ca2+ concentration as measured with the fluorescent Ca2+ indicator quin 2. 8-Bromo-cAMP and forskolin were inactive in this capacity. The Ca2+ ionophore A23187 increased the level of c-myc mRNA. Diacylglycerol and Ca2+ mediate the elevation of c-myc mRNA levels which is caused by platelet-derived growth factor and fibroblast growth factor (Kaibuchi, K., Tsuda, T., Kikuchi, A., Tanimoto, T., Yamashita, T., and Takai, Y. (1986) J. Biol. Chem. 261, 1187-1192). In contrast, the present results suggest that both cAMP and Ca2+ are involved in this PGE1-induced response in Swiss 3T3 cells.
- Subjects :
- DNA Replication
medicine.medical_specialty
Platelet-derived growth factor
medicine.medical_treatment
chemistry.chemical_element
Biology
Calcium
Fibroblast growth factor
Biochemistry
Mice
chemistry.chemical_compound
Internal medicine
Cyclic AMP
medicine
Animals
RNA, Messenger
Alprostadil
Protein kinase A
Molecular Biology
Cells, Cultured
Diacylglycerol kinase
Platelet-Derived Growth Factor
Forskolin
DNA synthesis
Growth factor
Oncogenes
Cell Biology
Fibroblasts
Kinetics
Endocrinology
chemistry
lipids (amino acids, peptides, and proteins)
Plasmids
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 261
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....238eab11b4465b431b5ae89c14c8f97f