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Arabidopsis stress-inducible gene for arginine decarboxylase AtADC2 is required for accumulation of putrescine in salt tolerance
- Source :
- Biochemical and biophysical research communications. 313(2)
- Publication Year :
- 2003
-
Abstract
- Arginine decarboxylase (ADC) catalyzes the first step of polyamine (PA) biosynthesis to produce putrescine (Put) from arginine (Arg). One of the 2 Arabidopsis ADC genes, AtADC2, is induced in response to salt stress causing the accumulation of free Put. To analyze the roles of stress-inducible AtADC2 gene and endogenous Put in stress tolerance, we isolated a Ds insertion mutant of AtADC2 gene (adc2-1) and characterized its phenotypes under salt stress. In the adc2-1 mutant, free Put content was reduced to about 25% of that in the control plants and did not increase under salt stress. Furthermore, the adc2-1 mutant was more sensitive to salt stress than the control plants. The stress sensitivity of adc2-1 was recovered by the addition of exogenous Put. These results indicate that endogenous Put plays an important role in salt tolerance in Arabidopsis. AtADC2 is a key gene for the production of Put under not only salinity conditions, but also normal conditions.
- Subjects :
- Arginine
Carboxy-Lyases
Acclimatization
Mutant
Biophysics
Arabidopsis
Endogeny
Biology
Sodium Chloride
Genes, Plant
Biochemistry
Gene Expression Regulation, Enzymologic
chemistry.chemical_compound
Biosynthesis
Gene Expression Regulation, Plant
Putrescine
Tissue Distribution
Molecular Biology
Arabidopsis Proteins
Histocytochemistry
Cell Biology
biology.organism_classification
Plants, Genetically Modified
Phenotype
chemistry
Enzyme Induction
Mutation
Polyamine
Arginine decarboxylase
Plant Structures
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 313
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....239127f530711d56c49943a2153d9dd2