Additional file 5 of Facilitating population genomics of non-model organisms through optimized experimental design for reduced representation sequencing

Additional file 5. Reduced representation sequencing (RRS) laboratory protocol based on the protocol from Peterson et al. (2012) [15]. The protocol is scaled for use with 192 samples and with restriction enzymes EcoRI and MspI; the reagent volumes can be scaled down/up to suit other sample numbers; if other enzymes are used, the respective reaction conditions must be adjusted.