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Quantifying CBM–Carbohydrate Interactions Using Microscale Thermophoresis
- Source :
- Methods in Molecular Biology ISBN: 9781071631508, Carbohydrate-Protein Interactions. Methods in Molecular Biology, Carbohydrate-Protein Interactions. Methods in Molecular Biology, pp.103-114, 2023, ⟨10.1007/978-1-0716-3151-5_7⟩
- Publication Year :
- 2023
- Publisher :
- Springer US, 2023.
-
Abstract
- International audience; Microscale thermophoresis (MST) is an emerging technology for studying a broad range of biomolecular interactions with a high sensitivity. The affinity constant can be obtained for a wide range of molecules within minutes based on reactions in microliters. Here we describe the application of MST in quantifying protein-carbohydrate interactions. A CBM3a and a CBM4 are titrated with insoluble substrate (cellulose nanocrystal) and soluble oligosaccharide (xylohexaose), respectively.
Details
- ISBN :
- 978-1-07-163150-8
- ISBNs :
- 9781071631508
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781071631508, Carbohydrate-Protein Interactions. Methods in Molecular Biology, Carbohydrate-Protein Interactions. Methods in Molecular Biology, pp.103-114, 2023, ⟨10.1007/978-1-0716-3151-5_7⟩
- Accession number :
- edsair.doi.dedup.....23b5083a12bcca1aa01b0fe61375ab77
- Full Text :
- https://doi.org/10.1007/978-1-0716-3151-5_7