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A novel method to detect Listeria monocytogenes via superparamagnetic lateral flow immunoassay
- Source :
- Analytical and Bioanalytical Chemistry. 407:529-535
- Publication Year :
- 2014
- Publisher :
- Springer Science and Business Media LLC, 2014.
-
Abstract
- A novel strip test system combining immunomagnetic separation with lateral flow immunoassay (LFIA) was established for the accurate detection of Listeria monocytogenes. In this system, a pair of matched monoclonal antibodies was used to construct a sandwich immunoassay, in which superparamagnetic particles were coupled with one of the antibodies as a labeled antibody to capture the target bacteria, while the other antibody was immobilized on the detection zone. After a 20-min reaction, the strips were analyzed by a novel instrument which could detect the magnetic signal of the immunocomplex in a magnetic field. Sensitivity evaluation showed that the limit of detection (LOD) of the superparamagnetic LFIA system for L. monocytogenes was 10(4) CFU/mL, which was at least one log lower than conventional LFIA. No cross-reaction was observed when Salmonella, Escherichia coli O157:H7, or three types of harmless Listeria strains were tested. Further evaluation with actual food samples indicated that the superparamagnetic LFIA system showed 100 % concordance with real-time PCR. Therefore, this novel superparamagnetic LFIA system could be used as a rapid, sensitive, and specific method for the detection of L. monocytogenes.
- Subjects :
- medicine.drug_class
Food Contamination
Cross Reactions
Escherichia coli O157
Real-Time Polymerase Chain Reaction
Immunomagnetic separation
Monoclonal antibody
medicine.disease_cause
Sensitivity and Specificity
Biochemistry
Analytical Chemistry
Listeria monocytogenes
Limit of Detection
Salmonella
medicine
Sandwich immunoassay
Immunoassay
Detection limit
biology
Immunomagnetic Separation
Chemistry
Antibodies, Monoclonal
Equipment Design
biology.organism_classification
Molecular biology
Food Microbiology
Listeria
Antibodies, Immobilized
Lateral flow immunoassay
Superparamagnetism
Subjects
Details
- ISSN :
- 16182650 and 16182642
- Volume :
- 407
- Database :
- OpenAIRE
- Journal :
- Analytical and Bioanalytical Chemistry
- Accession number :
- edsair.doi.dedup.....23c6bf259ff6ce40feb9a1e1a12779b2