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Isolation and characterization of microcystins from laboratory cultures and environmental samples ofMicrocystis aeruginosa and from an associated animal toxicosis
- Source :
- Natural Toxins. 3:50-57
- Publication Year :
- 1995
- Publisher :
- Wiley, 1995.
-
Abstract
- Six microcystins were identified in a laboratory culture of the cyanobacterium (blue-green alga) Microcystis aeruginosa PCC 7813 using high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) and mass spectrometry (LC-MS). The toxins were purified and further characterized by amino acid analysis and tandem mass spectrometry (MS-MS). The presence of the previously reported microcystin-LR and microcystin-LY was confirmed. Two further microcystins were characterized as microcystin-LW and microcystin-LF. Another two toxins were partially characterized and are believed to be an analog of microcystin-LR (molecular weight 1008) and microcystin-LM (molecular weight 969). Natural bloom material of M. aeruginosa collected from 2 reservoirs was found to have similar microcystin profiles using HPLC-DAD and LC-MS, indicating the widespread occurrence of these microcystin variants. In addition, the presence of 5 of the microcystins was confirmed in the rumen contents of a lamb by LC-MS and LC-MS-MS, providing the first report of microcystins identified in an animal suspected of being poisoned by cyanobacterial hepatotoxins.
- Subjects :
- Cyanobacteria
Microcystis
Rumen
Microcystins
Molecular Sequence Data
Sheep Diseases
Microcystin
Toxicology
Tandem mass spectrometry
Mass spectrometry
Peptides, Cyclic
Mass Spectrometry
Microbiology
Dogs
Phosphoprotein Phosphatases
polycyclic compounds
Animals
Water Pollutants
Microcystis aeruginosa
Amino Acid Sequence
Amino Acids
Chromatography, High Pressure Liquid
chemistry.chemical_classification
Sheep
biology
Hydrolysis
Liver Diseases
Hepatotoxin
Isolation (microbiology)
biology.organism_classification
Diode array
chemistry
Chemical and Drug Induced Liver Injury
Subjects
Details
- ISSN :
- 10569014
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- Natural Toxins
- Accession number :
- edsair.doi.dedup.....23c8ac8ba2a85fccb78e6c847c7b6881