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CD34 expression is regulated reciprocally with adhesion molecules in vascular endothelial cells in vitro
- Source :
- Europe PubMed Central, Scopus-Elsevier
- Publication Year :
- 1993
- Publisher :
- American Society of Hematology, 1993.
-
Abstract
- Freshly cultured vascular endothelial cells express the CD34 antigen in a diffuse cell surface pattern with some concentration on microvilli. Expression is downregulated with proliferation in continuous culture and undetectable after nine population doublings but can be maintained by restraining cell proliferation and promoting cell contact. Expression of CD34 at the antigen and mRNA levels on early passage cells is rapidly downregulated by interleukin-1 beta (IL-1 beta), interferon-gamma (INF-gamma), and tumor necrosis factor-alpha (TNF- alpha) under conditions in which these ligands upregulate the adhesion molecules: endothelial leukocyte adhesion molecule 1 (ELAM-1) and intracellular adhesion molecule 1 (ICAM-1). This reciprocal pattern of expression and the topographic distribution of CD34 molecules on the lumenal interdigitated microprocesses of adjacent endothelial cells in vivo suggest that CD34 might have a negative modulating role on adhesion functions of endothelia.
- Subjects :
- Endothelium
Immunology
Gene Expression
Antigens, CD34
Biology
Biochemistry
Interferon-gamma
Antigens, CD
E-selectin
medicine
Cell Adhesion
Humans
RNA, Messenger
Cell adhesion
Microvilli
Cell adhesion molecule
Tumor Necrosis Factor-alpha
Cell Membrane
Soluble cell adhesion molecules
Adhesion
Cell Biology
Hematology
Cell biology
Endothelial stem cell
medicine.anatomical_structure
biology.protein
Microscopy, Electron, Scanning
Neural cell adhesion molecule
Endothelium, Vascular
E-Selectin
Cell Adhesion Molecules
Cell Division
Interleukin-1
Subjects
Details
- ISSN :
- 15280020 and 00064971
- Volume :
- 81
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.doi.dedup.....23cc3d6b3931173a9eec1b5fccc1d28a