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A preliminary study on chitosan and gelatin polyelectrolyte complex cytocompatibility by cell cycle and apoptosis analysis
- Source :
- Biomaterials. 25(18)
- Publication Year :
- 2002
-
Abstract
- In this study, L929 cell adhesion, proliferation and apoptosis were investigated on chitosan (CS) and chitosan-gelatin (CS-Gel) membranes having different degrees of deacetylation (DD). The mitochondrial activity [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay results demonstrated that the DD played a key role in the L929 cell adhesion. Indeed, the higher the DD of a CS membrane, the stronger the cell adhesion. The cell cycle analysis showed that the CS surface inhibits most of the cells entering the cell cycle and the DD of CS had little effect on the cell cycle progression. On the other hand, this study also confirmed that CS, blended with Gel, induced L929 cells to enter the cell cycle, encouraged proliferation, decreased the degree of apoptosis on the CS membranes, and are comparable to the results from L929 cells on tissue culture plates. In brief, CS modified with Gel improves cytocompatibility by shielding the positively charged CS with a high charge density.
- Subjects :
- Materials science
food.ingredient
Macromolecular Substances
Biophysics
Cell Culture Techniques
Bioengineering
Apoptosis
Biocompatible Materials
Pilot Projects
Gelatin
Cell Line
Biomaterials
Chitosan
chemistry.chemical_compound
Electrolytes
Mice
food
Materials Testing
Cell Adhesion
Animals
Cell adhesion
Tissue Engineering
Cell Cycle
Membranes, Artificial
Adhesion
Cell cycle
Fibroblasts
Membrane
chemistry
Mechanics of Materials
Acetylation
Immunology
Ceramics and Composites
Cell Division
Subjects
Details
- ISSN :
- 01429612
- Volume :
- 25
- Issue :
- 18
- Database :
- OpenAIRE
- Journal :
- Biomaterials
- Accession number :
- edsair.doi.dedup.....23ec4d51bff5b8b85fd3d230c46c23d4