Additional file 1 of Mitochondrial supplementation of Sus scrofa metaphase II oocytes alters DNA methylation and gene expression profiles of blastocysts

Additional file 1: Figure S1. Schematic representation of the production of autologous mICSI-derived blastocysts. Figure S2. WGBS data of Sus scrofa oocytes (Oc) and ICSI- (IB) and mICSI-derived blastocysts (MB) analysed by the 100-CpG window method and visualized by SeqMonk. Figure S3. Comparative analysis of WGBS data sets from Sus scrofa oocytes (Oc), ICSI- (IB) and mICSI-derived blastocysts (MB). Figure S4. DNA methylation status in each Sus scrofa chromosome. Figure S5. Longitudinal comparison of DMRs to capture differences in the DNA methylation reprogramming process as a result of mtDNA supplementation. Figure S6. Methylation status of imprinted genes (A) KCNQ1, (B) GNAS and (C) MEST in Sus scrofa oocytes (Oc) and blastocysts (IB and MB). Figure S7. PCA of Sus scrofa ICSI- (red) and mICSI- (green) derived blastocyst RNAseq data. Figure S8. Volcano plots displaying differential gene expression between Sus scrofa ICSI- and mICSI-derived blastocysts. Figure S9. Expression of (A) 52 DEGs between ICSI- and mICSI-derived blastocysts (Table 2); (B) genes catalysing cytosine methylation and demethylation; and (C) genes involved in embryonic genome activation presented by heatmap. Figure S10. Expression of genes of interest in Sus scrofa ICSI- and mICSI-derived blastocysts presented by box plots.