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An Aspergillus nidulans endo-β-1,3-glucanase exhibited specific catalytic features and was used to prepare 3-O-β-cellobiosyl-d-glucose and 3-O-β-gentiobiosyl-d-glucose with high antioxidant activity from barley β-glucan and laminarin, respectively
- Source :
- International journal of biological macromolecules. 186
- Publication Year :
- 2021
-
Abstract
- An endo-β-1,3(4)-glucanase AnENG16A from Aspergillus nidulans shows distinctive catalytic features for hydrolysis of β-glucans. AnENG16A hydrolyzed Eisenia bicyclis laminarin to mainly generate 3-O-β-gentiobiosyl-d-glucose and hydrolyzed barley β-glucan to mainly produce 3-O-β-cellobiosyl-d-glucose. Using molecular exclusion chromatography, we isolated and purified 3-O-β-cellobiosyl-d-glucose and 3-O-β-gentiobiosyl-d-glucose, respectively, from AnENG16A-hydrolysate of barley β-glucan and E. bicyclis laminarin. Further study reveals that 3-O-β-cellobiosyl-d-glucose had 8.99-fold higher antioxidant activity than barley β-glucan and 3-O-β-gentiobiosyl-d-glucose exhibited 43.0% higher antioxidant activity than E. bicyclis laminarin. Notably, 3-O-β-cellobiosyl-d-glucose and 3-O-β-gentiobiosyl-d-glucose exhibited 148.9% and 116.0% higher antioxidant activity than laminaritriose, respectively, indicating that β-1,4-linkage or -1,6-linkage at non-reducing end of β-glucotrioses had enhancing effect on antioxidant activity compared to β-1,3-linkage. Furthermore, 3-O-β-cellobiosyl-d-glucose showed 237.9% higher antioxidant activity than cellotriose, and laminarin showed 5.06-fold higher antioxidant activity than barley β-glucan, indicating that β-1,4-linkage at reducing end of β-glucans or oligosaccharides resulted in decrease of antioxidant activity compared to β-1,3-linkage.
- Subjects :
- Antioxidant
Cellobiose
beta-Glucans
medicine.medical_treatment
Biochemistry
Antioxidants
Aspergillus nidulans
Catalysis
Substrate Specificity
Hydrolysis
Laminarin
chemistry.chemical_compound
Structure-Activity Relationship
Picrates
Structural Biology
D-Glucose
medicine
Molecular Biology
Glucans
Glucan
chemistry.chemical_classification
biology
Molecular Structure
Glucan Endo-1,3-beta-D-Glucosidase
Biphenyl Compounds
Hordeum
General Medicine
Glucanase
biology.organism_classification
Glucose
chemistry
Eisenia
Subjects
Details
- ISSN :
- 18790003
- Volume :
- 186
- Database :
- OpenAIRE
- Journal :
- International journal of biological macromolecules
- Accession number :
- edsair.doi.dedup.....2436244a9a05d1fb7a7a1f9cfe5d64d6