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Oral vaccination with LcrV from Yersinia pestis KIM delivered by live attenuated Salmonellaenterica serovar Typhimurium elicits a protective immune response against challenge with Yersinia pseudotuberculosis and Yersinia enterocolitica
- Source :
- Vaccine. 27:5363-5370
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- The use of live recombinant attenuated Salmonella vaccines (RASV) synthesizing Yersinia proteins is a promising approach for controlling infection by Yersinia species. In this study, we constructed attenuated Salmonella strains which synthesize a truncated form of LcrV, LcrV196 and evaluated the immune response and protective efficacy elicited by these strains in mice against two other major species of Yersinia: Yersinia pseudotuberculosis and Yersinia enterocolitica. Surprisingly, we found that the RASV strain alone was sufficient to afford nearly full protection against challenge with Y. pseudotuberculosis, indicating the likelihood that Salmonella produces immunogenic cross-protective antigens. In contrast, lcrV196 expression was required for protection against challenge with Y. enterocolitica strain 8081, but was not sufficient to achieve significant protection against challenge with Y. enterocolitica strain WA, which expressed a divergent form of lcrV. Nevertheless, we are encouraged by these findings to continue pursuing our long-term goal of developing a single vaccine to protect against all three human pathogenic species of Yersinia.
- Subjects :
- DNA, Bacterial
Pore Forming Cytotoxic Proteins
Salmonella typhimurium
Yersinia Infections
Yersinia pestis
Administration, Oral
Cross Reactions
Yersinia
Vaccines, Attenuated
Article
Microbiology
Mice
Animals
Yersinia pseudotuberculosis
LcrV
Yersinia enterocolitica
Antigens, Bacterial
Vaccines, Synthetic
General Veterinary
General Immunology and Microbiology
biology
Public Health, Environmental and Occupational Health
biology.organism_classification
Antibodies, Bacterial
Virology
Bacterial vaccine
Infectious Diseases
Salmonella enterica
Bacterial Vaccines
bacteria
Molecular Medicine
Female
Subjects
Details
- ISSN :
- 0264410X
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Vaccine
- Accession number :
- edsair.doi.dedup.....244ee12a7e259b1ee3696f610bde3f83