Supplementary Figures S1-S7 from LGR5-Targeted Antibody–Drug Conjugate Eradicates Gastrointestinal Tumors and Prevents Recurrence

Supplementary Fig. S1- Coomassie gel of anti-LGR5 mAb and anti-LGR5 ADCs. Supplementary Fig. S2- Confocal image of HEK293T cells with stable over-expression of hLGR4. Supplementary Fig. S3- Anti-LGR5 ADC binding assay and cytotoxicity of free MMAE. Supplementary Fig. S4- Anti-LGR5 mAb crossreactivity with mouse LGR5 and images of intestinal organoids treated with anti-LGR5 ADC or free MMAE. Supplementary Fig. S5- Representative images of LoVo xenograft tumors in mice from each treatment cohort and graph of average body weight. Supplementary Fig. S6- H&E staining of intestinal tissue sections. Supplementary Fig. S7. Images of Lovo xenograft tumor recurrence and weight.