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Citrus CitNAC62 cooperates with CitWRKY1 to participate in citric acid degradation via up-regulation of CitAco3
- Source :
- Journal of Experimental Botany
- Publication Year :
- 2017
- Publisher :
- Oxford University Press, 2017.
-
Abstract
- Two novel transcription factors, CitNAC62 and CitWRKY1, were involved in citric acid degradation in citrus fruit, potentially via enhanced expression of CitAco3.<br />Citric acid is the predominant organic acid of citrus fruit. Degradation of citric acid occurs during fruit development, influencing fruit acidity. Associations of CitAco3 transcripts and citric acid degradation have been reported for citrus fruit. Here, transient overexpression of CitAco3 significantly reduced the citric acid content of citrus leaves and fruits. Using dual luciferase assays, it was shown that CitNAC62 and CitWRKY1 could transactivate the promoter of CitAco3. Subcellular localization results showed that CitWRKY1 was located in the nucleus and CitNAC62 was not. Yeast two-hybrid analysis and bimolecular fluorescence complementation (BiFC) assays indicated that the two differently located transcription factors could interact with each other. Furthermore, BiFC showed that the protein–protein interaction occurred only in the nucleus, indicating the potential mobility of CitNAC62 in plant cells. A synergistic effect on citrate content was observed between CitNAC62 and CitWRKY1. Transient overexpression of CitNAC62 or CitWRKY1 led to significantly lower citrate content in citrus fruit. The combined expression of CitNAC62 and CitWRKY1 resulted in lower citrate content compared with the expression of CitNAC62 or CitWRKY1 alone. The transcript abundance of CitAco3 was consistent with the citrate content. Thus, we propose that a complex of CitWRKY1 and CitNAC62 contributes to citric acid degradation in citrus fruit, potentially via modulation of CitAco3.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Transcriptional Activation
Citrus
Physiology
Plant Science
01 natural sciences
Aconitase
03 medical and health sciences
Bimolecular fluorescence complementation
chemistry.chemical_compound
stomatognathic system
CitWRKY1
Gene Expression Regulation, Plant
Gene expression
Luciferase
transcriptional regulation
Plant Proteins
chemistry.chemical_classification
food and beverages
citric acid
Plant cell
Research Papers
CitNAC62
Yeast
030104 developmental biology
protein–protein interaction
Biochemistry
chemistry
Growth and Development
Citric acid
010606 plant biology & botany
Organic acid
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 14602431 and 00220957
- Volume :
- 68
- Issue :
- 13
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Botany
- Accession number :
- edsair.doi.dedup.....2460d83df9706e05869897cc2c3c1ed2