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Chlorella diet alters mitochondrial cardiolipin contents differentially in organs of Danio rerio analyzed by a lipidomics approach
- Source :
- PLoS ONE, Vol 13, Iss 3, p e0193042 (2018), PLoS ONE
- Publication Year :
- 2018
- Publisher :
- Public Library of Science (PLoS), 2018.
-
Abstract
- The zebrafish (Danio rerio) is an important and widely used vertebrate model organism for the study of human diseases which include disorders caused by dysfunctional mitochondria. Mitochondria play an essential role in both energy metabolism and apoptosis, which are mediated through a mitochondrial phospholipid cardiolipin (CL). In order to examine the cardiolipin profile in the zebrafish model, we developed a CL analysis platform by using liquid chromatography-mass spectrometry (LC-MS). Meanwhile, we tested whether chlorella diet would alter the CL profile in the larval fish, and in various organs of the adult fish. The results showed that chlorella diet increased the chain length of CL in larval fish. In the adult zebrafish, the distribution patterns of CL species were similar between the adult brain and eye tissues, and between the heart and muscles. Interestingly, monolyso-cardiolipin (MLCL) was not detected in brain and eyes but found in other examined tissues, indicating a different remodeling mechanism to maintain the CL integrity. While the adult zebrafish were fed with chlorella for four weeks, the CL distribution showed an increase of the species of saturated acyl chains in the brain and eyes, but a decrease in the other organs. Moreover, chlorella diet led to a decrease of MLCL percentage in organs except the non-MLCL-containing brain and eyes. The CL analysis in the zebrafish provides an important tool for studying the mechanism of mitochondria diseases, and may also be useful for testing medical regimens targeting against the Barth Syndrome.
- Subjects :
- 0301 basic medicine
ved/biology.organism_classification_rank.species
lcsh:Medicine
Chlorella
Mitochondrion
Biochemistry
chemistry.chemical_compound
Medicine and Health Sciences
Cardiolipin
lcsh:Science
Musculoskeletal System
Zebrafish
Energy-Producing Organelles
Phospholipids
Multidisciplinary
biology
Muscles
Eukaryota
Heart
Animal Models
Eye Muscles
Lipids
Mitochondria
Cell biology
Experimental Organism Systems
Osteichthyes
Vertebrates
Animal Nutritional Physiological Phenomena
Female
Anatomy
Cellular Structures and Organelles
Research Article
Cardiolipins
Ocular Anatomy
Danio
Bioenergetics
Research and Analysis Methods
03 medical and health sciences
Model Organisms
Ocular System
Lipidomics
Animals
Model organism
Nutrition
Cardiac Muscles
030102 biochemistry & molecular biology
ved/biology
lcsh:R
Organisms
Biology and Life Sciences
Cell Biology
biology.organism_classification
Animal Feed
Diet
Fish
030104 developmental biology
chemistry
Apoptosis
Barth Syndrome
Cardiovascular Anatomy
Eyes
lcsh:Q
Energy Metabolism
Head
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....2476693e86e8ab1d713a2bc31e4d9eeb