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Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET
- Source :
- Genome Medicine
- Publication Year :
- 2010
-
Abstract
- Anchored ChromPET, a technique to capture and interrogate targeted sequences in the genome, has been developed to identify chromosomal aberrations and define breakpoints. Using this method, we could define the BCR-ABL1 translocation DNA breakpoint to a base-pair resolution in Philadelphia chromosome-positive samples. This DNA-based method is highly sensitive and can detect the fusion junction using samples from which it is hard to obtain RNA or cells where the RNA expression has been silenced.
- Subjects :
- Genetics
0303 health sciences
Systems biology
Breakpoint
RNA
Method
Chromosomal translocation
Computational biology
Biology
Proteomics
Genome
Human genetics
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
chemistry
030220 oncology & carcinogenesis
hemic and lymphatic diseases
Molecular Medicine
Genetics(clinical)
Molecular Biology
Genetics (clinical)
DNA
030304 developmental biology
Subjects
Details
- ISSN :
- 1756994X
- Volume :
- 2
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Genome medicine
- Accession number :
- edsair.doi.dedup.....248c108499760ce628efa708848dd8cd