Isolation and characterization of 10 microsatellite loci in Cneorum tricoccon (Cneoraceae), a Mediterranean relict plant

Premise of the study: The main aim of this study was to isolate and characterize microsatellite loci in Cneorum tricoccon (Cneoraceae), a Mediterranean shrub relict of the early Tertiary, which inhabits western Mediterranean islands and coasts. Microsatellites will be useful for investigating biogeography and landscape genetics across the species distribution range, including current or past gene flow. Methods and Results: Seventeen microsatellite loci were characterized, of which 10 were polymorphic and amplified for a total of 56 alleles in three populations of C. tricoccon. The markers revealed average coefficients of expected heterozygosity (H e = 0.425), observed heterozygosity (H o = 0.282), and inbreeding coefficient value per population (F IS = 0.408). Conclusions: These microsatellite primers will potentially be useful in the study of population and landscape genetics, conservation status of isolated populations, island-continental distribution, current or historical movements between populations, and in the investigation of the consequences of dispersal mechanisms of these plants. © 2012 Botanical Society of America.
Financial support was provided by Global Change International Laboratory (CSIC-PUC, LincGlobal), the Spanish Ministry of Science and Innovation (grants VULGLO CGL2010-22180-C03-03 and Consolider Montes CSD2008_00040), and by the Comunidad de Madrid grant REMEDINAL 2 (CM-S2009/AMB-1783)