Back to Search
Start Over
Enhancement of Environmental Hazard Degradation in the Presence of Lignin: a Proteomics Study
- Source :
- Scientific Reports, Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Proteomics studies of fungal systems have progressed dramatically based on the availability of more fungal genome sequences in recent years. Different proteomics strategies have been applied toward characterization of fungal proteome and revealed important gene functions and proteome dynamics. Presented here is the application of shot-gun proteomic technology to study the bio-remediation of environmental hazards by white-rot fungus. Lignin, a naturally abundant component of the plant biomass, is discovered to promote the degradation of Azo dye by white-rot fungus Irpex lacteus CD2 in the lignin/dye/fungus system. Shotgun proteomics technique was used to understand degradation mechanism at the protein level for the lignin/dye/fungus system. Our proteomics study can identify about two thousand proteins (one third of the predicted white-rot fungal proteome) in a single experiment, as one of the most powerful proteomics platforms to study the fungal system to date. The study shows a significant enrichment of oxidoreduction functional category under the dye/lignin combined treatment. An in vitro validation is performed and supports our hypothesis that the synergy of Fenton reaction and manganese peroxidase might play an important role in DR5B dye degradation. The results could guide the development of effective bioremediation strategies and efficient lignocellulosic biomass conversion.
- Subjects :
- Proteomics
0301 basic medicine
030106 microbiology
Irpex lacteus
lcsh:Medicine
Gene Expression
Lignocellulosic biomass
Lignin
complex mixtures
Article
Hazardous Substances
03 medical and health sciences
chemistry.chemical_compound
Biotransformation
Manganese peroxidase
Botany
lcsh:Science
Shotgun proteomics
Multidisciplinary
biology
lcsh:R
fungi
Fungi
Computational Biology
food and beverages
biology.organism_classification
Biodegradation, Environmental
030104 developmental biology
chemistry
Biochemistry
Proteome
lcsh:Q
Azo Compounds
Oxidation-Reduction
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....24b0b8c8cb6bc834e446102b58555a78
- Full Text :
- https://doi.org/10.1038/s41598-017-10132-4