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Enhancement of Environmental Hazard Degradation in the Presence of Lignin: a Proteomics Study

Authors :
Honglu Zhao
Yanbing Cheng
Shangxian Xie
Muzi Li
Su Sun
Hongbo Yu
Xiaoyu Zhang
Joshua S. Yuan
Xiaotong Li
Susie Y. Dai
Source :
Scientific Reports, Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
Publication Year :
2017
Publisher :
Springer Science and Business Media LLC, 2017.

Abstract

Proteomics studies of fungal systems have progressed dramatically based on the availability of more fungal genome sequences in recent years. Different proteomics strategies have been applied toward characterization of fungal proteome and revealed important gene functions and proteome dynamics. Presented here is the application of shot-gun proteomic technology to study the bio-remediation of environmental hazards by white-rot fungus. Lignin, a naturally abundant component of the plant biomass, is discovered to promote the degradation of Azo dye by white-rot fungus Irpex lacteus CD2 in the lignin/dye/fungus system. Shotgun proteomics technique was used to understand degradation mechanism at the protein level for the lignin/dye/fungus system. Our proteomics study can identify about two thousand proteins (one third of the predicted white-rot fungal proteome) in a single experiment, as one of the most powerful proteomics platforms to study the fungal system to date. The study shows a significant enrichment of oxidoreduction functional category under the dye/lignin combined treatment. An in vitro validation is performed and supports our hypothesis that the synergy of Fenton reaction and manganese peroxidase might play an important role in DR5B dye degradation. The results could guide the development of effective bioremediation strategies and efficient lignocellulosic biomass conversion.

Details

ISSN :
20452322
Volume :
7
Database :
OpenAIRE
Journal :
Scientific Reports
Accession number :
edsair.doi.dedup.....24b0b8c8cb6bc834e446102b58555a78
Full Text :
https://doi.org/10.1038/s41598-017-10132-4