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Pilot Study Evaluating a Rat Model of Radiation-induced Erectile Dysfunction Using an Image-guided Microirradiator
- Source :
- Urology. 85(5)
- Publication Year :
- 2014
-
Abstract
- Objective To establish a feasible rat model of radiation-induced erectile dysfunction after targeted prostate irradiation using an image-guided irradiation unit specially designed for small-animal radiation research. Methods The X-RAD 225Cx research platform was used in the present study. We first performed quality assurance testing using a rat cadaver. After confirming dosimetry, 24 age-matched, young, adult, male rats were assigned to sham radiation or radiation to the prostate with doses of 15, 20, or 25 Gy. To confirm appropriate prostate irradiation, physiological erectile function was evaluated using intracavernous pressure (ICP) measurements with cavernous nerve electrical stimulation at 9 weeks after radiotherapy. Each animal was weighed at the time of ICP measurement. In addition, we investigated the cyclic guanosine monophosphate level in the penile cavernosa using a commercial enzyme-linked immunosorbent assay kit. Results Quality assurance results confirmed the accuracy of the irradiation technique. Dose-dependent decreases in ICP in irradiated rats were observed without major toxicity. No difference in body weight was noted among the experimental groups. Cyclic guanosine monophosphate levels were significantly decreased in the group that received 25 Gy compared with the age-matched sham-irradiated group. Conclusion High-precision imaging and targeting capabilities provided by the micro-IGRT platform enable us to develop a reproducible animal model of radiation-induced erectile dysfunction in prostate cancer research.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Urology
medicine.medical_treatment
Stimulation
Pilot Projects
Radiation Dosage
Rats, Sprague-Dawley
Prostate cancer
chemistry.chemical_compound
Erectile Dysfunction
Prostate
medicine
Dosimetry
Animals
Radiation Injuries
Cyclic guanosine monophosphate
Cyclic GMP
business.industry
medicine.disease
Rats
Radiation therapy
Disease Models, Animal
medicine.anatomical_structure
Erectile dysfunction
chemistry
Toxicity
business
Penis
Subjects
Details
- ISSN :
- 15279995
- Volume :
- 85
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Urology
- Accession number :
- edsair.doi.dedup.....24e73fa6bbbbaefb6eaf9f077630b7b0