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Detection of low-frequency DNA variants by targeted sequencing of the Watson and Crick strands
- Source :
- Nat Biotechnol
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Identification and quantification of low-frequency mutations remain challenging despite improvements in the baseline error rate of next-generation sequencing technologies. Here, we describe a method, termed SaferSeqS, that addresses these challenges by (1) efficiently introducing identical molecular barcodes in the Watson and Crick strands of template molecules and (2) enriching target sequences with strand-specific PCR. The method achieves high sensitivity and specificity and detects variants at frequencies below 1 in 100,000 DNA template molecules with a background mutation rate of 100-fold.
- Subjects :
- 0303 health sciences
Mutation rate
Dna template
Biomedical Engineering
Word error rate
Bioengineering
Genomics
Molecular Structure of Nucleic Acids: A Structure for Deoxyribose Nucleic Acid
Computational biology
Biology
Dna variants
Applied Microbiology and Biotechnology
Genome
Article
DNA sequencing
03 medical and health sciences
0302 clinical medicine
Molecular Medicine
030217 neurology & neurosurgery
030304 developmental biology
Biotechnology
Subjects
Details
- ISSN :
- 15461696 and 10870156
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Nature Biotechnology
- Accession number :
- edsair.doi.dedup.....24fb58fbee1abcff3474200148e4d6dd