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Aquaporin 5 promotes the proliferation and migration of human gastric carcinoma cells
- Source :
- Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 34(3)
- Publication Year :
- 2012
-
Abstract
- Aquaporin 5 (AQP5) promotes the progression and invasion of several cancers, but its role in the tumorigenesis of human gastric carcinoma (GC) has not been clearly defined. Here, we investigated the potential functions of AQP5 in the proliferation and migration of human GC. RT-PCR and western blotting were used to detect the expression of AQP5 in human GC cell lines. Immunohistochemistry was applied to evaluate the expression of AQP5 in human GC tissues and corresponding normal tissues. Following ectopic overexpression of AQP5 or inhibition of AQP5 by its inhibitor, acetazolamide (AZA), cell proliferation and migration of AGS cells were analyzed by MTT assay, colony formation assay, and wound healing assay. Heterogeneous expression of AQP5 mRNA and protein was observed in human GC cell lines MKN45, MKN28, AGS, and SGC7901. AQP5 was up-regulated in GC tissues in comparison to corresponding normal tissues. AQP5 protein was mainly localized in the cell membrane. Overexpression of AQP5 was correlated with enhanced lymph node metastasis. In vitro, overexpression of AQP5 notably enhanced, while inhibition of AQP5 by AZA significantly attenuated the proliferation and migration of AGS cells. Our data indicate that AQP5 may play an important role in the tumorigenesis and progression of human GC and suggest that AQP5 is a potential therapeutic target against GC.
- Subjects :
- Male
Blotting, Western
Aquaporin
Apoptosis
Enzyme-Linked Immunosorbent Assay
Biology
Adenocarcinoma
medicine.disease_cause
Flow cytometry
Colony-Forming Units Assay
Immunoenzyme Techniques
Cell Movement
Stomach Neoplasms
medicine
Tumor Cells, Cultured
Humans
MTT assay
Neoplasm Invasiveness
Cell Proliferation
Neoplasm Staging
Wound Healing
medicine.diagnostic_test
Cell growth
Stomach
General Medicine
Middle Aged
Flow Cytometry
Molecular biology
Aquaporin 5
Blot
Acetazolamide
Cell culture
Gastric Mucosa
Lymphatic Metastasis
Cancer research
Immunohistochemistry
Anticonvulsants
Female
Carcinogenesis
Subjects
Details
- ISSN :
- 14230380
- Volume :
- 34
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
- Accession number :
- edsair.doi.dedup.....250fc78afdb203944194f5c67efd0739