Additional file 3 of Hydroxychloroquine synergizes with the PI3K inhibitor BKM120 to exhibit antitumor efficacy independent of autophagy

Additional file 3: Figure S3. The effect of Spautin-1 on the autophagy and cytotoxity caused by BKM120. Representative images of autophagosome puncta (A) in SKOV-3 and MKN-1 treated with BKM120 (1 ��M), Spautin-1 (10 ��M), alone or in combination for 24 h, and the quantification (B). Scale bars, 20 ��m. (C) Cell viability of HBC-5 cells after treated with different concentrations of BKM120 alone or in combination with 10 ��M Spautin-1 for 72 h. (D) Colony forming ability of HBC-5 cells after treated with Spautin-1 or/and BKM120. Graph of colony quantification is shown in (E). Graphs are normalized to 100% per treatment and shown as mean �� SD from three independent experiments; ns: p > 0.05; **p