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The control of paramyxovirus genome hexamer length and mRNA editing
- Source :
- RNA. 24:461-467
- Publication Year :
- 2018
- Publisher :
- Cold Spring Harbor Laboratory, 2018.
-
Abstract
- The unusual ability of a human parainfluenza virus type 2 (hPIV2) nucleoprotein point mutation (NPQ202A) to strongly enhance minigenome replication was found to depend on the absence of a functional, internal element of the bipartite replication promoter (CRII). This point mutation allows relatively robust CRII-minus minigenome replication in a CRII-independent manner, under conditions in which NPwt is essentially inactive. The nature of the amino acid at position 202 apparently controls whether viral RNA-dependent RNA polymerase (vRdRp) can, or cannot, initiate RNA synthesis in a CRII-independent manner. By repressing genome synthesis when vRdRp cannot correctly interact with CRII, gln202 of N, the only residue of the RNA-binding groove that contacts a nucleotide base in the N-RNA, acts as a gatekeeper for wild-type (CRII-dependent) RNA synthesis. This ensures that only hexamer-length genomes are replicated, and that the critical hexamer phase of the cis-acting mRNA editing sequence is maintained.
- Subjects :
- 0301 basic medicine
Genome, Viral
Biology
Random hexamer
Virus Replication
Genome
Cell Line
03 medical and health sciences
chemistry.chemical_compound
Cricetinae
Report
RNA polymerase
Animals
Humans
Point Mutation
Nucleotide
RNA, Messenger
Promoter Regions, Genetic
Molecular Biology
chemistry.chemical_classification
Binding Sites
Point mutation
RNA-Dependent RNA Polymerase
Parainfluenza Virus 2, Human
Nucleoprotein
Cell biology
Amino acid
Nucleoproteins
030104 developmental biology
chemistry
RNA editing
RNA-Binding Motifs
RNA, Viral
RNA Editing
Subjects
Details
- ISSN :
- 14699001 and 13558382
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- RNA
- Accession number :
- edsair.doi.dedup.....2527a32564bc08691a54cad8ebe450d6