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High level expression of β-mannanase (RmMan5A) in Pichia pastoris for partially hydrolyzed guar gum production
- Source :
- International Journal of Biological Macromolecules. 105:1171-1179
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Partially hydrolyzed guar gum (PHGG), an important supplemental dietary fiber, has been used as food ingredient in many industries. In this study, a novel β-mannanase gene (RmMan5A) from Rhizomucor miehei was successfully expressed in Pichia pastoris and subjected for PHGG production. Enzyme activity of fermentation supernatant reached 85,200UmL-1 after 168h high cell density fermentation. The purified RmMan5A exhibited the highest enzyme activity at pH 7.0 and 65°C. RmMan5A was then employed for guar gum hydrolysis and PHGG obtained demonstrated a weight-average molecular weight (Mw) of 2.5×104Da. Total dietary fiber accounted 90.6% of PHGG and 24.9% (w/w) of PHGG were identified as manno-oligosaccharides with degree of polymerization
- Subjects :
- 0106 biological sciences
0301 basic medicine
Gene Expression
Galactans
01 natural sciences
Biochemistry
Pichia
Pichia pastoris
Mannans
03 medical and health sciences
Hydrolysis
chemistry.chemical_compound
Ingredient
Structural Biology
010608 biotechnology
Plant Gums
Rhizomucor
Molecular Biology
Ethanol precipitation
Chromatography
Guar gum
biology
Chemistry
Temperature
beta-Mannosidase
General Medicine
Hydrogen-Ion Concentration
biology.organism_classification
Enzyme assay
Molecular Weight
030104 developmental biology
Galactose
Fermentation
biology.protein
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 105
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....252ed34832edb894c5096e0dccf5ded9